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• W2010025906 abstract "RNA was extracted from human gestational membranes and villous placental tissue following spontaneous delivery (n = 15) or elective caesarean section (n = 15) at term. The samples were subjected to Northern analysis, using a 2 kb cDNA probe for peroxisome proliferator activated receptor (PPAR)-gamma. The mRNA was detectable in all choriodecidual and villous placental samples, irrespective of mode of delivery, but was only rarely detectable in the amnion. The JEG3 choriocarcinoma cell line also expressed PPARgamma. In order to evaluate PPAR mediated transcriptional activation in JEG3 cells, the cells were transfected with pTK-PPREx3-luc, a PPAR response element (PPRE) containing luciferase reporter construct. Subsequent treatment with 10 microm 15-deoxy-delta(12,14)prostaglandin J(2)(15dPGJ(2)) resulted in an eight-fold stimulation of luciferase production relative to controls transfected with the same construct lacking the PPRE. This stimulation was concentration-dependent. These results suggest roles for PPARgamma and its ligand in lipid, steroid and inflammatory mediator homeostasis and in remodelling of gestational tissues." @default.
• W2010025906 created "2016-06-24" @default.
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• W2010025906 date "2000-05-01" @default.
• W2010025906 modified "2023-09-27" @default.
• W2010025906 title "The 15-Deoxy-Δ12,14-prostaglandin J2Receptor, Peroxisome Proliferator Activated Receptor-γ (PPARγ) is Expressed in Human Gestational Tissues and is Functionally Active in JEG3 Choriocarcinoma Cells" @default.
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• W2010025906 doi "https://doi.org/10.1053/plac.1999.0485" @default.
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