Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010048257> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2010048257 endingPage "650" @default.
- W2010048257 startingPage "644" @default.
- W2010048257 abstract "To improve ATP production from adenine, we optimized cultivation and reaction conditions for the ATP producing strain, Corynebacterium ammoniagenes KY13510. In the conventional method, 28% NH4OH has been used both to adjust pH during cultivation and reaction, and to provide nitrogen for cell growth. In the ATP-producing reaction, high concentrations of inorganic phosphate and magnesium ion are needed, which form magnesium ammonium phosphate (MgNH4PO4) precipitate. To keep inorganic phosphate and magnesium ions soluble in the reaction mixture, it was indispensable to add phytic acid as a chelating agent of divalent metal ions. Under such conditions, 37 mg/ml (61.2 mM) ATP was accumulated in 13 h (Appl. Microbiol. Biotechnol. 21, 143 1985). If ammonium ion was depleted from the reaction mixture to avoid MgNH4 PO4 formation, we expected that there was no need to add phytic acid and ATP accumulation might be improved. Therefore, we obtained the cultured broth of C. ammoniagenes KY13510 strain with low ammonium ion content (less than 1 mg/ml as NH3) by the method that a part of alkali solution (28% NH4OH) for pH control was replaced with 10 N KOH. Using this culture broth, ATP producing reaction was done in 2-liter jar fermentor, controlling the pH of the reaction mixture with 10 N KOH. Under these conditions, the rate of ATP accumulation improved greatly, and 70.6 mg/ml (117 mM) ATP was accumulated in 28 h. The molar conversion ratio from adenine to ATP was about 82%. Phytic acid was slightly inhibitory to ATP formation under these ammonium-limited conditions." @default.
- W2010048257 created "2016-06-24" @default.
- W2010048257 creator A5075465229 @default.
- W2010048257 creator A5083061750 @default.
- W2010048257 date "2001-01-01" @default.
- W2010048257 modified "2023-09-23" @default.
- W2010048257 title "ATP Production from Adenine by a Self-coupling Enzymatic Process: High-level Accumulation under Ammonium-limited Conditions" @default.
- W2010048257 cites W1674507293 @default.
- W2010048257 cites W1971944396 @default.
- W2010048257 cites W1986211308 @default.
- W2010048257 cites W1988922615 @default.
- W2010048257 cites W1999594601 @default.
- W2010048257 cites W2094588780 @default.
- W2010048257 cites W2122346488 @default.
- W2010048257 cites W2141034126 @default.
- W2010048257 cites W2163586642 @default.
- W2010048257 cites W2392702874 @default.
- W2010048257 doi "https://doi.org/10.1271/bbb.65.644" @default.
- W2010048257 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11330681" @default.
- W2010048257 hasPublicationYear "2001" @default.
- W2010048257 type Work @default.
- W2010048257 sameAs 2010048257 @default.
- W2010048257 citedByCount "14" @default.
- W2010048257 countsByYear W20100482572012 @default.
- W2010048257 countsByYear W20100482572013 @default.
- W2010048257 countsByYear W20100482572015 @default.
- W2010048257 countsByYear W20100482572016 @default.
- W2010048257 countsByYear W20100482572021 @default.
- W2010048257 countsByYear W20100482572023 @default.
- W2010048257 crossrefType "journal-article" @default.
- W2010048257 hasAuthorship W2010048257A5075465229 @default.
- W2010048257 hasAuthorship W2010048257A5083061750 @default.
- W2010048257 hasBestOaLocation W20100482571 @default.
- W2010048257 hasConcept C100544194 @default.
- W2010048257 hasConcept C112243037 @default.
- W2010048257 hasConcept C13965031 @default.
- W2010048257 hasConcept C171357686 @default.
- W2010048257 hasConcept C178790620 @default.
- W2010048257 hasConcept C179104552 @default.
- W2010048257 hasConcept C181199279 @default.
- W2010048257 hasConcept C185592680 @default.
- W2010048257 hasConcept C2777132085 @default.
- W2010048257 hasConcept C2777751223 @default.
- W2010048257 hasConcept C2778036281 @default.
- W2010048257 hasConcept C2779870022 @default.
- W2010048257 hasConcept C2780560099 @default.
- W2010048257 hasConcept C543218039 @default.
- W2010048257 hasConcept C55493867 @default.
- W2010048257 hasConceptScore W2010048257C100544194 @default.
- W2010048257 hasConceptScore W2010048257C112243037 @default.
- W2010048257 hasConceptScore W2010048257C13965031 @default.
- W2010048257 hasConceptScore W2010048257C171357686 @default.
- W2010048257 hasConceptScore W2010048257C178790620 @default.
- W2010048257 hasConceptScore W2010048257C179104552 @default.
- W2010048257 hasConceptScore W2010048257C181199279 @default.
- W2010048257 hasConceptScore W2010048257C185592680 @default.
- W2010048257 hasConceptScore W2010048257C2777132085 @default.
- W2010048257 hasConceptScore W2010048257C2777751223 @default.
- W2010048257 hasConceptScore W2010048257C2778036281 @default.
- W2010048257 hasConceptScore W2010048257C2779870022 @default.
- W2010048257 hasConceptScore W2010048257C2780560099 @default.
- W2010048257 hasConceptScore W2010048257C543218039 @default.
- W2010048257 hasConceptScore W2010048257C55493867 @default.
- W2010048257 hasIssue "3" @default.
- W2010048257 hasLocation W20100482571 @default.
- W2010048257 hasLocation W20100482572 @default.
- W2010048257 hasOpenAccess W2010048257 @default.
- W2010048257 hasPrimaryLocation W20100482571 @default.
- W2010048257 hasRelatedWork W117816802 @default.
- W2010048257 hasRelatedWork W1988210237 @default.
- W2010048257 hasRelatedWork W2001180299 @default.
- W2010048257 hasRelatedWork W2001818108 @default.
- W2010048257 hasRelatedWork W2019704516 @default.
- W2010048257 hasRelatedWork W2020590802 @default.
- W2010048257 hasRelatedWork W2086632188 @default.
- W2010048257 hasRelatedWork W2132378380 @default.
- W2010048257 hasRelatedWork W2242024575 @default.
- W2010048257 hasRelatedWork W2750566048 @default.
- W2010048257 hasVolume "65" @default.
- W2010048257 isParatext "false" @default.
- W2010048257 isRetracted "false" @default.
- W2010048257 magId "2010048257" @default.
- W2010048257 workType "article" @default.