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• W2010048458 abstract "The rotational properties of two eosin-labelled fatty acids of different alkyl chain length have been studied in large multilamellar dimyristoylphosphatidylcholine vesicles. The location of the probes at the surface region were ascertained by quenching experiments using a hydrophilic divalent cation solubilized in the aqueous phase (Cu2+) and a hydrophobic aromatic aniline (N,N-dimethylaniline) associated with the lipid. Phosphorescence anisotropy measurements reveal that above the phospholipid phase transition the polarization of eosin luminescence decays monoexponentially in the micro-to-millisecond time range, while below the phase transition a biexponential decay is observed. A model is proposed which attributes the time constants to two separate motions, discrete jumps or 'flipping' of the eosin moiety within restricted boundaries and long-axis rotation. The value of the time-independent term changes with probe position and temperature and reflects orientational constraints imposed by lipid-chromophore interactions. The implications of these results for the study of protein rotations in membranes are discussed." @default.
• W2010048458 created "2016-06-24" @default.
• W2010048458 creator A5034685558 @default.
• W2010048458 creator A5077048047 @default.
• W2010048458 date "1986-05-01" @default.
• W2010048458 modified "2023-10-16" @default.
• W2010048458 title "The microsecond rotational motions of eosin-labelled fatty acids in multilamellar vesicles" @default.
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• W2010048458 doi "https://doi.org/10.1016/0005-2736(86)90101-x" @default.
• W2010048458 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2421777" @default.
• W2010048458 hasPublicationYear "1986" @default.
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