Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010050447> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2010050447 endingPage "83" @default.
- W2010050447 startingPage "76" @default.
- W2010050447 abstract "The spin trap 5,5-dimethyl-1-pyrroline-1-oxide (DMPO) alone, as well as DMPO or N-tert-butyl-α-phenylnitrone (PBN) in the presence of excess dimethyl sulfoxide (Me2SO), have been used as spin trapping systems for the detection of hydroxyl radical. However, the instability of DMPO and many of its corresponding spin-trapped adducts has limited the usefulness of this spin trap, particularly in biological systems. Spin trapping of multiple free radicals by the PBN/Me2SO system may undermine the sensitivity of this method to detect small, yet biologically significant amounts of hydroxyl radical. The present study was undertaken to select a spin trapping system with greater sensitivity and selectivity toward ·OH than DMPO, DMPO/Me2SO, or PBN/Me2SO. We report that α-hydroxyethyl radical, resulting from the reaction of photolytically generated ·OH with excess ethanol is spin trapped by 4-pyridyl-1-oxide-N-tert-butylnitrone (4-POBN) with a second-order rate constant nearly 10-fold greater than that for DMPO or PBN. In contrast to DMPO spin-trapped ad-ducts, the α-hydroxyethyl radical adduct of 4-POBN, 4-POBN-CH(CH3)OH, is resistant to reduction by superoxide, even in the presence of cysteine. The efficiency of spin trapping and the marked stability of the resulting spin-trapped adduct confer a high degree of sensitivity and demonstrate the potential application of 4-POBN/ETOH toward the detection of hydroxyl radical in biological systems." @default.
- W2010050447 created "2016-06-24" @default.
- W2010050447 creator A5044177815 @default.
- W2010050447 creator A5050719909 @default.
- W2010050447 creator A5057501339 @default.
- W2010050447 creator A5061937758 @default.
- W2010050447 creator A5066396391 @default.
- W2010050447 creator A5076075120 @default.
- W2010050447 creator A5076093109 @default.
- W2010050447 creator A5077076538 @default.
- W2010050447 date "1994-02-01" @default.
- W2010050447 modified "2023-09-26" @default.
- W2010050447 title "A Kinetic Approach to the Selection of a Sensitive Spin Trapping System for the Detection of Hydroxyl Radical" @default.
- W2010050447 doi "https://doi.org/10.1006/abio.1994.1085" @default.
- W2010050447 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8203741" @default.
- W2010050447 hasPublicationYear "1994" @default.
- W2010050447 type Work @default.
- W2010050447 sameAs 2010050447 @default.
- W2010050447 citedByCount "109" @default.
- W2010050447 countsByYear W20100504472012 @default.
- W2010050447 countsByYear W20100504472013 @default.
- W2010050447 countsByYear W20100504472014 @default.
- W2010050447 countsByYear W20100504472015 @default.
- W2010050447 countsByYear W20100504472016 @default.
- W2010050447 countsByYear W20100504472017 @default.
- W2010050447 countsByYear W20100504472018 @default.
- W2010050447 countsByYear W20100504472019 @default.
- W2010050447 countsByYear W20100504472020 @default.
- W2010050447 countsByYear W20100504472022 @default.
- W2010050447 countsByYear W20100504472023 @default.
- W2010050447 crossrefType "journal-article" @default.
- W2010050447 hasAuthorship W2010050447A5044177815 @default.
- W2010050447 hasAuthorship W2010050447A5050719909 @default.
- W2010050447 hasAuthorship W2010050447A5057501339 @default.
- W2010050447 hasAuthorship W2010050447A5061937758 @default.
- W2010050447 hasAuthorship W2010050447A5066396391 @default.
- W2010050447 hasAuthorship W2010050447A5076075120 @default.
- W2010050447 hasAuthorship W2010050447A5076093109 @default.
- W2010050447 hasAuthorship W2010050447A5077076538 @default.
- W2010050447 hasConcept C108204754 @default.
- W2010050447 hasConcept C121332964 @default.
- W2010050447 hasConcept C139066938 @default.
- W2010050447 hasConcept C148898269 @default.
- W2010050447 hasConcept C178790620 @default.
- W2010050447 hasConcept C185592680 @default.
- W2010050447 hasConcept C18903297 @default.
- W2010050447 hasConcept C2776778087 @default.
- W2010050447 hasConcept C2777924906 @default.
- W2010050447 hasConcept C2778452553 @default.
- W2010050447 hasConcept C2778815226 @default.
- W2010050447 hasConcept C62520636 @default.
- W2010050447 hasConcept C75473681 @default.
- W2010050447 hasConcept C86803240 @default.
- W2010050447 hasConcept C93391505 @default.
- W2010050447 hasConceptScore W2010050447C108204754 @default.
- W2010050447 hasConceptScore W2010050447C121332964 @default.
- W2010050447 hasConceptScore W2010050447C139066938 @default.
- W2010050447 hasConceptScore W2010050447C148898269 @default.
- W2010050447 hasConceptScore W2010050447C178790620 @default.
- W2010050447 hasConceptScore W2010050447C185592680 @default.
- W2010050447 hasConceptScore W2010050447C18903297 @default.
- W2010050447 hasConceptScore W2010050447C2776778087 @default.
- W2010050447 hasConceptScore W2010050447C2777924906 @default.
- W2010050447 hasConceptScore W2010050447C2778452553 @default.
- W2010050447 hasConceptScore W2010050447C2778815226 @default.
- W2010050447 hasConceptScore W2010050447C62520636 @default.
- W2010050447 hasConceptScore W2010050447C75473681 @default.
- W2010050447 hasConceptScore W2010050447C86803240 @default.
- W2010050447 hasConceptScore W2010050447C93391505 @default.
- W2010050447 hasIssue "1" @default.
- W2010050447 hasLocation W20100504471 @default.
- W2010050447 hasLocation W20100504472 @default.
- W2010050447 hasOpenAccess W2010050447 @default.
- W2010050447 hasPrimaryLocation W20100504471 @default.
- W2010050447 hasRelatedWork W1995340760 @default.
- W2010050447 hasRelatedWork W2011578453 @default.
- W2010050447 hasRelatedWork W2014925765 @default.
- W2010050447 hasRelatedWork W2032846370 @default.
- W2010050447 hasRelatedWork W2188567860 @default.
- W2010050447 hasRelatedWork W2382067821 @default.
- W2010050447 hasRelatedWork W2419315897 @default.
- W2010050447 hasRelatedWork W2582746614 @default.
- W2010050447 hasRelatedWork W2592691545 @default.
- W2010050447 hasRelatedWork W2746385347 @default.
- W2010050447 hasVolume "217" @default.
- W2010050447 isParatext "false" @default.
- W2010050447 isRetracted "false" @default.
- W2010050447 magId "2010050447" @default.
- W2010050447 workType "article" @default.