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- W2010051339 abstract "The present study describes a live-cell biosensor, suitable for general evaluation of adhesion qualities of different substrates. It is based on NIH/3T3 fibroblast cell line stably expressing fusion fluorescently tagged proteins mCherry-vinculin and GFP-tensin as quantifiable markers for assessment not only of focal but also of fibrillar contacts. Four measurable parameters - spreading, polarization and development of focal and fibrillar adhesions were used to standardize the adhesion of biosensor cells after plating on five substrates of natural origin - fibronectin, vitronectin, laminin-111, laminin-521 and collagen type I. The obtained set of values (adhesion quality map) were utilized to describe the default biosensor behavior and as a standard for evaluation of surface biocompatibility of materials with unknown adhesive properties. To demonstrate the applicability of the biosensor we studied two PDMS-based artificial materials. The results demonstrated the superior adhesive properties of the poly(acrylic acid)-containing polymer (PDMS-PAA) over that of poly(vinyl pyrrolidone) copolymer (PDMS-PVP), and pointed out the formation of focal adhesions as a parameter for possible further improvements." @default.
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- W2010051339 date "2009-01-01" @default.
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- W2010051339 title "Imaging circuit assembly in the developing retina" @default.
- W2010051339 doi "https://doi.org/10.1016/j.neures.2009.09.1492" @default.
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