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- W2010053259 abstract "Carbonic anhydrase (CA) was purified from the unicellular green alga Chlamydomonas reinhardii, and the purity of the preparation was established by gradient gel electrophoresis. The purified enzyme exhibited a MW of 165 000 and contained 6 atoms of Zn. The subunit MW, as determined by dodecyl sulfate electrophoresis, was 27 000. These results are consistent with a quarternary structure which is hexameric, each monomer containing 1 g atom of Zn. Like spinach CA, and in contrast to other oligomeric plant CAs, a sulfhydryl reducing agent is not needed to stabilize the enzyme. CO2-hydrase activity was inhibited by both acetazolamide (I50 = 7.8 × 10−9M) and sulfanilamide (I50 = 1.3 × 10−5M), as well as by certain inorganic anions. The purified enzyme showed relatively weak esterase activity with p-nitrophenyl acetate but was an extremely effective esterase with 2-hydroxy-5-nitro-α-toluenesulfonic acid sultone as the substrate. Both esterase activities could be completely inhibited by adding acetazolamide. In its gross structural characteristics, the C. reinhardii enzyme resembles the CAs from higher plants. However, in its esterase activity and the inhibition by sulfonamides it is markedly different from plant CAs and bears more resemblance to erythrocyte CAs." @default.
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- W2010053259 date "1980-01-01" @default.
- W2010053259 modified "2023-09-23" @default.
- W2010053259 title "Purification and properties of carbonic anhydrase from Chlamydomonas reinhardii" @default.
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- W2010053259 doi "https://doi.org/10.1016/s0031-9422(00)83913-8" @default.
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