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• W2010054211 abstract "Abstract In the present paper we have characterized the ability of an irradiation with visible light of BrdUrd-containing chromosomes to induce sister-chromatid exchanges (SCEs). The experimental approach has include the use of post-treatments with oxygen, caffeine or benzamide during the pre-replicative period of the cell cycle in Allium cepa L. root-meristem cells exposed to visible light (VL) during early G1. Cells post-treated with benzamide immediately after VL showed higher SCE levels than those post-treated with either caffeine or oxygen. SCE-inducing DNA damage, repaired by a benzamide-sensitive step, appeared to be eliminated with a very fast kinetics. Thus, 15 min between the end of VL exposure and the beginning of benzamide treatment were enough for the cells to exhibit normal SCE levels. However, for the case of oxygen, that time was shown to be 2–3 h. Since these agents presumably act at different levels of the repair process, our results suggests that different SCE-inducing lesions were produced by VL exposure and/or that they were repaired at different times throughout the G1 phase. Finally, when cells irradiated at the beginning of the G1 phase were treated with either caffeine or benzamide during their G1/S traverse, SCE levels were significantly increased. These results were consistent with the occurrenceof a benzamide- and caffeine-sensitive survey mechanism, acting at the G1/S border, that would tend to reduce the SCE-inducing DNA damage before the S-phase." @default.
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• W2010054211 date "1988-01-01" @default.
• W2010054211 modified "2023-09-28" @default.
• W2010054211 title "Sister-chromatid exchange (SCE) elimination kinetics in BrdUrd-treated cells irradiated with visible light" @default.
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• W2010054211 doi "https://doi.org/10.1016/0027-5107(88)90147-9" @default.
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