Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010054491> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W2010054491 endingPage "544" @default.
- W2010054491 startingPage "539" @default.
- W2010054491 abstract "Abstract During the growth of a uracil-requiring mutant of S. cerevisiae under conditions of limiting uracil concentration in the growth medium, derepression of aspartate transcarbamylase (ATCase) was accompanied by a progressive loss of its sensitivity to inhibition by UTP, one of the final products in the pyrimidine biosynthetic chain. The loss in feedback inhibition could be prevented or reversed by adding excess uracil to the medium in which the cells were growing. If fully repressed and fully derepressed cells were mixed prior to extracting the enzyme, the feedback inhibition of the resulting enzyme preparation was considerably higher than that predicted on the basis of the sensitivity to UTP of the repressed and derepressed enzymes alone. No significant enhancement of feedback inhibition was noted if the crude extracts themselves were mixed (i.e., after extraction from the repressed and derepressed cells). Virtually the same effect was noted if the derepressed cells were mixed with a mutant strain whose ATCase lacked the regulatory site; cells of this mutant strain possess very high intracellular levels of UTP. The hypothesis that UTP was the intracellular factor responsible for stabilizing the feedback site of the repressed enzyme was confirmed by the finding that UTP, alone among all the pyrimidines, pyrimidine nucleotides, and purine nucleotides tested, could stabilize the feedback site during extraction. On the other hand, UTP had no effect in stabilizing the feedback of a semipurified ATCase preparation against inactivation by heat. It thus appears that UTP has three distinct roles in regulation of pyrimidine biosynthesis in the yeast cell: it is the agent both of feedback inhibition of ATCase activity and of repression of its biosynthesis and it stabilizes the regulatory site of the enzyme." @default.
- W2010054491 created "2016-06-24" @default.
- W2010054491 creator A5030555932 @default.
- W2010054491 creator A5069579102 @default.
- W2010054491 creator A5076996005 @default.
- W2010054491 date "1969-02-01" @default.
- W2010054491 modified "2023-09-26" @default.
- W2010054491 title "On the loss of feedback inhibition of yeast aspartate transcarbamylase during derepression of pyrimidine biosynthesis" @default.
- W2010054491 cites W1497003308 @default.
- W2010054491 cites W1808527256 @default.
- W2010054491 cites W2021051542 @default.
- W2010054491 cites W2154615269 @default.
- W2010054491 doi "https://doi.org/10.1016/0003-9861(69)90212-4" @default.
- W2010054491 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/5772966" @default.
- W2010054491 hasPublicationYear "1969" @default.
- W2010054491 type Work @default.
- W2010054491 sameAs 2010054491 @default.
- W2010054491 citedByCount "15" @default.
- W2010054491 crossrefType "journal-article" @default.
- W2010054491 hasAuthorship W2010054491A5030555932 @default.
- W2010054491 hasAuthorship W2010054491A5069579102 @default.
- W2010054491 hasAuthorship W2010054491A5076996005 @default.
- W2010054491 hasConcept C104317684 @default.
- W2010054491 hasConcept C150194340 @default.
- W2010054491 hasConcept C181199279 @default.
- W2010054491 hasConcept C185592680 @default.
- W2010054491 hasConcept C186310378 @default.
- W2010054491 hasConcept C197248121 @default.
- W2010054491 hasConcept C200159599 @default.
- W2010054491 hasConcept C2776476023 @default.
- W2010054491 hasConcept C2779222958 @default.
- W2010054491 hasConcept C2779321679 @default.
- W2010054491 hasConcept C547475151 @default.
- W2010054491 hasConcept C553450214 @default.
- W2010054491 hasConcept C55493867 @default.
- W2010054491 hasConcept C73766848 @default.
- W2010054491 hasConcept C86803240 @default.
- W2010054491 hasConceptScore W2010054491C104317684 @default.
- W2010054491 hasConceptScore W2010054491C150194340 @default.
- W2010054491 hasConceptScore W2010054491C181199279 @default.
- W2010054491 hasConceptScore W2010054491C185592680 @default.
- W2010054491 hasConceptScore W2010054491C186310378 @default.
- W2010054491 hasConceptScore W2010054491C197248121 @default.
- W2010054491 hasConceptScore W2010054491C200159599 @default.
- W2010054491 hasConceptScore W2010054491C2776476023 @default.
- W2010054491 hasConceptScore W2010054491C2779222958 @default.
- W2010054491 hasConceptScore W2010054491C2779321679 @default.
- W2010054491 hasConceptScore W2010054491C547475151 @default.
- W2010054491 hasConceptScore W2010054491C553450214 @default.
- W2010054491 hasConceptScore W2010054491C55493867 @default.
- W2010054491 hasConceptScore W2010054491C73766848 @default.
- W2010054491 hasConceptScore W2010054491C86803240 @default.
- W2010054491 hasIssue "2" @default.
- W2010054491 hasLocation W20100544911 @default.
- W2010054491 hasLocation W20100544912 @default.
- W2010054491 hasOpenAccess W2010054491 @default.
- W2010054491 hasPrimaryLocation W20100544911 @default.
- W2010054491 hasRelatedWork W1965942630 @default.
- W2010054491 hasRelatedWork W1967971144 @default.
- W2010054491 hasRelatedWork W1989341321 @default.
- W2010054491 hasRelatedWork W1998202807 @default.
- W2010054491 hasRelatedWork W2003092871 @default.
- W2010054491 hasRelatedWork W2032848924 @default.
- W2010054491 hasRelatedWork W2058453049 @default.
- W2010054491 hasRelatedWork W2115039509 @default.
- W2010054491 hasRelatedWork W2156002611 @default.
- W2010054491 hasRelatedWork W2170552338 @default.
- W2010054491 hasVolume "129" @default.
- W2010054491 isParatext "false" @default.
- W2010054491 isRetracted "false" @default.
- W2010054491 magId "2010054491" @default.
- W2010054491 workType "article" @default.