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- W2010056951 abstract "A sensitive radiochemical assay is described for the assay of acetoacetyl-CoA synthetase activity in cytosolic extracts. Enzyme activity is measured by the incorporation of 14C from acetoacetate into acetyl carnitine as mediated by acetoacetyl-CoA synthetase, endogenous acetoacetyl-CoA thiolase, and exogenous carnitine acetyl transferase. Separation of 14C-labeled reactants from 14C-labeled acetyl carnitine is achieved by cation-exchange chromatography. The assay is sensitive with less than 10 pmol of product readily detected. Acetoacetyl-CoA synthetase activity was measured in human fibroblasts, 0.12 nmol min−1 mg cytosolic protein−1, and was found to be more than two orders of magnitude below the activity level of acetoacetyl-CoA synthetase in rat liver cytosol, 18.4 nmol min−1 mg cytosolic protein−1. An HPLC method is also described for the purification of [3-14C]acetoacetate." @default.
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- W2010056951 date "1985-09-01" @default.
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- W2010056951 title "A radiochemical assay for acetoacetyl-CoA synthetase" @default.
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- W2010056951 doi "https://doi.org/10.1016/0003-2697(85)90583-4" @default.
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