FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010064429> ?p ?o ?g. }

• W2010064429 endingPage "279" @default.
• W2010064429 startingPage "272" @default.
• W2010064429 abstract "A sensitive, nonradioactive method was developed to measure cellular levels of dimethylallyl diphosphate (DMAPP), a central intermediate of isoprenoid metabolism in nature. The assay is based on the hydrolysis of DMAPP in acid to the volatile hydrocarbon isoprene (2-methyl-1,3-butadiene), with subsequent analysis of isoprene by headspace gas chromatography with reduction gas detection. In the assay, cell samples are directly acidified with 4 M H(2)SO(4) in sealed reaction vials. Therefore, there is no need to extract metabolites, purify them, and keep them stable prior to analysis, and degradative enzymatic activities are destroyed. DMAPP levels of 23 +/- 4 nmol (g fresh weight)(-1) [ca. 85 nmol (g dry weight)(-1)] and 80 +/- 14 nmol (g fresh weight)(-1) [ca. 296 nmol (g dry weight)(-1)] were measured in dark- and light-adapted leaves of Populus deltoides (Eastern cottonwood), respectively. Evidence is presented to show that DMAPP is the major leaf metabolite giving rise to isoprene following acid hydrolysis. DMAPP levels in Bacillus subtilis and Saccharomyces cerevisiae were determined to be 40.8 +/- 16.7 pmol (OD(600))(-1) [ca. 638 pmol (mg dry weight)(-1)] and 6.3 +/- 3.7 pmol (OD(600))(-1) [ca. 139 pmol (mg dry weight)(-1)], respectively. The method should be suitable for any cell or tissue type and isolated cellular organelles." @default.
• W2010064429 created "2016-06-24" @default.
• W2010064429 creator A5014572912 @default.
• W2010064429 creator A5016460313 @default.
• W2010064429 creator A5046926845 @default.
• W2010064429 creator A5068047363 @default.
• W2010064429 date "2001-05-01" @default.
• W2010064429 modified "2023-09-27" @default.
• W2010064429 title "Nonradioactive Assay for Cellular Dimethylallyl Diphosphate" @default.
• W2010064429 cites W1513705939 @default.
• W2010064429 cites W1538564037 @default.
• W2010064429 cites W1560945520 @default.
• W2010064429 cites W1570124205 @default.
• W2010064429 cites W1674845299 @default.
• W2010064429 cites W1854003852 @default.
• W2010064429 cites W1883228224 @default.
• W2010064429 cites W1936032545 @default.
• W2010064429 cites W1965628579 @default.
• W2010064429 cites W1968948383 @default.
• W2010064429 cites W1983915009 @default.
• W2010064429 cites W1984079793 @default.
• W2010064429 cites W1993236551 @default.
• W2010064429 cites W1997857169 @default.
• W2010064429 cites W2002316663 @default.
• W2010064429 cites W2002491503 @default.
• W2010064429 cites W2004915791 @default.
• W2010064429 cites W2013287304 @default.
• W2010064429 cites W2037247242 @default.
• W2010064429 cites W2038878107 @default.
• W2010064429 cites W2058144544 @default.
• W2010064429 cites W2071124826 @default.
• W2010064429 cites W2074972947 @default.
• W2010064429 cites W2081950192 @default.
• W2010064429 cites W2086834274 @default.
• W2010064429 cites W2102155348 @default.
• W2010064429 cites W2115667821 @default.
• W2010064429 cites W2138939726 @default.
• W2010064429 cites W2151467394 @default.
• W2010064429 cites W2159958980 @default.
• W2010064429 cites W2176603852 @default.
• W2010064429 cites W2191540396 @default.
• W2010064429 cites W2487680245 @default.
• W2010064429 cites W2741770376 @default.
• W2010064429 doi "https://doi.org/10.1006/abio.2001.5079" @default.
• W2010064429 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11355861" @default.
• W2010064429 hasPublicationYear "2001" @default.
• W2010064429 type Work @default.
• W2010064429 sameAs 2010064429 @default.
• W2010064429 citedByCount "49" @default.
• W2010064429 countsByYear W20100644292012 @default.
• W2010064429 countsByYear W20100644292013 @default.
• W2010064429 countsByYear W20100644292014 @default.
• W2010064429 countsByYear W20100644292015 @default.
• W2010064429 countsByYear W20100644292016 @default.
• W2010064429 countsByYear W20100644292017 @default.
• W2010064429 countsByYear W20100644292019 @default.
• W2010064429 countsByYear W20100644292020 @default.
• W2010064429 countsByYear W20100644292021 @default.
• W2010064429 countsByYear W20100644292022 @default.
• W2010064429 countsByYear W20100644292023 @default.
• W2010064429 crossrefType "journal-article" @default.
• W2010064429 hasAuthorship W2010064429A5014572912 @default.
• W2010064429 hasAuthorship W2010064429A5016460313 @default.
• W2010064429 hasAuthorship W2010064429A5046926845 @default.
• W2010064429 hasAuthorship W2010064429A5068047363 @default.
• W2010064429 hasConcept C123460561 @default.
• W2010064429 hasConcept C150668497 @default.
• W2010064429 hasConcept C15920480 @default.
• W2010064429 hasConcept C178790620 @default.
• W2010064429 hasConcept C185592680 @default.
• W2010064429 hasConcept C2777477808 @default.
• W2010064429 hasConcept C2780959689 @default.
• W2010064429 hasConcept C31903555 @default.
• W2010064429 hasConcept C43617362 @default.
• W2010064429 hasConcept C521977710 @default.
• W2010064429 hasConcept C55493867 @default.
• W2010064429 hasConcept C59822182 @default.
• W2010064429 hasConcept C62231903 @default.
• W2010064429 hasConcept C86803240 @default.
• W2010064429 hasConcept C94412978 @default.
• W2010064429 hasConceptScore W2010064429C123460561 @default.
• W2010064429 hasConceptScore W2010064429C150668497 @default.
• W2010064429 hasConceptScore W2010064429C15920480 @default.
• W2010064429 hasConceptScore W2010064429C178790620 @default.
• W2010064429 hasConceptScore W2010064429C185592680 @default.
• W2010064429 hasConceptScore W2010064429C2777477808 @default.
• W2010064429 hasConceptScore W2010064429C2780959689 @default.
• W2010064429 hasConceptScore W2010064429C31903555 @default.
• W2010064429 hasConceptScore W2010064429C43617362 @default.
• W2010064429 hasConceptScore W2010064429C521977710 @default.
• W2010064429 hasConceptScore W2010064429C55493867 @default.
• W2010064429 hasConceptScore W2010064429C59822182 @default.
• W2010064429 hasConceptScore W2010064429C62231903 @default.
• W2010064429 hasConceptScore W2010064429C86803240 @default.
• W2010064429 hasConceptScore W2010064429C94412978 @default.
• W2010064429 hasIssue "2" @default.
• W2010064429 hasLocation W20100644291 @default.
• W2010064429 hasLocation W20100644292 @default.

• next