FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010067741> ?p ?o ?g. }

• W2010067741 endingPage "13" @default.
• W2010067741 startingPage "5" @default.
• W2010067741 abstract "Cultured skin substitutes have been used as adjunctive therapies in the treatment of burns and chronic wounds, but they are limited by lack of a vascular plexus. This deficiency leads to greater time for vascularization compared with native skin autografts and contributes to graft failure. Genetic modification of cultured skin substitutes to enhance vascularization could hypothetically lead to improved wound healing. To address this hypothesis, human keratinocytes were genetically modified by transduction with a replication incompetent retrovirus to overexpress vascular endothelial growth factor, a specific and potent mitogen for endothelial cells. Cultured skin substitutes consisting of collagen-glycosaminoglycan substrates inoculated with human fibroblasts and either vascular endothelial growth factor-modified or control keratinocytes were prepared, and were cultured in vitro for 21 d. Northern blot analysis demonstrated enhanced expression of vascular endothelial growth factor mRNA in genetically modified keratinocytes and in cultured skin substitutes prepared with modified cells. Furthermore, the vascular endothelial growth factor-modified cultured skin substitutes secreted greatly elevated levels of vascular endothelial growth factor protein throughout the entire culture period. The bioactivity of vascular endothelial growth factor protein secreted by the genetically modified cultured skin substitutes was demonstrated using a microvascular endothelial cell growth assay. Vascular endothelial growth factor-modified and control cultured skin substitutes were grafted to full-thickness wounds on athymic mice, and elevated vascular endothelial growth factor mRNA expression was detected in the modified grafts for at least 2 wk after surgery. Vascular endothelial growth factor-modified grafts exhibited increased numbers of dermal blood vessels and decreased time to vascularization compared with controls. These results indicate that genetic modification of keratinocytes in cultured skin substitutes can lead to increased vascular endothelial growth factor expression, which could prospectively improve vascularization of cultured skin substitutes for wound healing applications." @default.
• W2010067741 created "2016-06-24" @default.
• W2010067741 creator A5015488107 @default.
• W2010067741 creator A5019178531 @default.
• W2010067741 creator A5024094891 @default.
• W2010067741 creator A5084845278 @default.
• W2010067741 date "2000-01-01" @default.
• W2010067741 modified "2023-09-27" @default.
• W2010067741 title "Enhanced Vascularization of Cultured Skin Substitutes Genetically Modified to Overexpress Vascular Endothelial Growth Factor11The authors declared in writing to have no conflict of interest." @default.
• W2010067741 cites W1550364580 @default.
• W2010067741 cites W1969442477 @default.
• W2010067741 cites W1971248512 @default.
• W2010067741 cites W1972141220 @default.
• W2010067741 cites W1992380657 @default.
• W2010067741 cites W2013038209 @default.
• W2010067741 cites W2013396957 @default.
• W2010067741 cites W2013641657 @default.
• W2010067741 cites W2015789556 @default.
• W2010067741 cites W2017627019 @default.
• W2010067741 cites W2026393226 @default.
• W2010067741 cites W2035371785 @default.
• W2010067741 cites W2041609936 @default.
• W2010067741 cites W2042756665 @default.
• W2010067741 cites W2042761977 @default.
• W2010067741 cites W2043852940 @default.
• W2010067741 cites W2045247659 @default.
• W2010067741 cites W2055887720 @default.
• W2010067741 cites W2056146626 @default.
• W2010067741 cites W2058340155 @default.
• W2010067741 cites W2062594120 @default.
• W2010067741 cites W2066991122 @default.
• W2010067741 cites W2074029723 @default.
• W2010067741 cites W2076105533 @default.
• W2010067741 cites W2076310543 @default.
• W2010067741 cites W2082723727 @default.
• W2010067741 cites W2085951747 @default.
• W2010067741 cites W2088437212 @default.
• W2010067741 cites W2092246905 @default.
• W2010067741 cites W2093498435 @default.
• W2010067741 cites W2141046053 @default.
• W2010067741 cites W2444172977 @default.
• W2010067741 doi "https://doi.org/10.1046/j.1523-1747.2000.00824.x" @default.
• W2010067741 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10620108" @default.
• W2010067741 hasPublicationYear "2000" @default.
• W2010067741 type Work @default.
• W2010067741 sameAs 2010067741 @default.
• W2010067741 citedByCount "113" @default.
• W2010067741 countsByYear W20100677412012 @default.
• W2010067741 countsByYear W20100677412013 @default.
• W2010067741 countsByYear W20100677412014 @default.
• W2010067741 countsByYear W20100677412015 @default.
• W2010067741 countsByYear W20100677412016 @default.
• W2010067741 countsByYear W20100677412017 @default.
• W2010067741 countsByYear W20100677412018 @default.
• W2010067741 countsByYear W20100677412019 @default.
• W2010067741 countsByYear W20100677412020 @default.
• W2010067741 countsByYear W20100677412021 @default.
• W2010067741 countsByYear W20100677412022 @default.
• W2010067741 countsByYear W20100677412023 @default.
• W2010067741 crossrefType "journal-article" @default.
• W2010067741 hasAuthorship W2010067741A5015488107 @default.
• W2010067741 hasAuthorship W2010067741A5019178531 @default.
• W2010067741 hasAuthorship W2010067741A5024094891 @default.
• W2010067741 hasAuthorship W2010067741A5084845278 @default.
• W2010067741 hasBestOaLocation W20100677411 @default.
• W2010067741 hasConcept C103041312 @default.
• W2010067741 hasConcept C123012128 @default.
• W2010067741 hasConcept C146285616 @default.
• W2010067741 hasConcept C15215337 @default.
• W2010067741 hasConcept C167734588 @default.
• W2010067741 hasConcept C170493617 @default.
• W2010067741 hasConcept C202751555 @default.
• W2010067741 hasConcept C203014093 @default.
• W2010067741 hasConcept C2775960820 @default.
• W2010067741 hasConcept C2777025900 @default.
• W2010067741 hasConcept C2780269544 @default.
• W2010067741 hasConcept C2780394083 @default.
• W2010067741 hasConcept C502942594 @default.
• W2010067741 hasConcept C55493867 @default.
• W2010067741 hasConcept C86803240 @default.
• W2010067741 hasConceptScore W2010067741C103041312 @default.
• W2010067741 hasConceptScore W2010067741C123012128 @default.
• W2010067741 hasConceptScore W2010067741C146285616 @default.
• W2010067741 hasConceptScore W2010067741C15215337 @default.
• W2010067741 hasConceptScore W2010067741C167734588 @default.
• W2010067741 hasConceptScore W2010067741C170493617 @default.
• W2010067741 hasConceptScore W2010067741C202751555 @default.
• W2010067741 hasConceptScore W2010067741C203014093 @default.
• W2010067741 hasConceptScore W2010067741C2775960820 @default.
• W2010067741 hasConceptScore W2010067741C2777025900 @default.
• W2010067741 hasConceptScore W2010067741C2780269544 @default.
• W2010067741 hasConceptScore W2010067741C2780394083 @default.
• W2010067741 hasConceptScore W2010067741C502942594 @default.
• W2010067741 hasConceptScore W2010067741C55493867 @default.
• W2010067741 hasConceptScore W2010067741C86803240 @default.
• W2010067741 hasIssue "1" @default.
• W2010067741 hasLocation W20100677411 @default.
• W2010067741 hasLocation W20100677412 @default.

• next