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- W2010082134 abstract "Abstract Out of nine phage-resistant mutants ofPseudomonas syringaepv.morsprunorumstrain C28, seven were avirulent to cherry cv. Napoleon, and all induced a hypersensitive response on tobacco. Of the three mutants resistant to phage A7, all contained rough lipopolysaccharide (LPS), as did four of the six mutants resistant to phage A15; the remaining two appeared to produce smooth LPS. Outer membrane preparations obtained by EDTA extraction from six of the nine mutants prevented hypersensitive necrosis in tobacco and caused silvering in bean leaves, at concentrations comparable with those of EDTA extract from strain C28, but preparations from two rough phage A15-resistant mutants gave weak prevention of hypersensitive necrosis, and the extract from one mutant resistant to phage A7 gave poor silvering as well as weak prevention of necrosis. The biological activity of EDTA extracts from all mutants was enhanced by treatment with calcium and magnesium ions. Rough LPS preparations from three mutants prevented hypersensitive necrosis in tobacco at 3–16μgml-1, and caused silvering in bean at 4–11μgml-1. Smooth LPS of strain C28 was inactive, but was converted to an active form after removal of the side-chain by treatment with phage A7." @default.
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- W2010082134 date "1996-01-01" @default.
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- W2010082134 title "Outer membrane and rough lipopolysaccharide preparations of mutants ofPseudomonas syringaepv.morsprunorumC28 prevent hypersensitive necrosis and cause silveringin planta" @default.
- W2010082134 doi "https://doi.org/10.1006/pmpp.1996.0002" @default.
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