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- W2010096286 abstract "The manganese peroxidase produced by Phanerochaete chrysosporium, which catalyzes the oxidation of Mn(2+) to Mn(3+), is easily inactivated by the hydrogen peroxide (H2O2) presented in the reaction. We attempted to increase H2O2 resistance by the conformational stabilization around the H2O2-binding pocket. Based on its structural model, engineering of oxidizable Met273 located near the pocket to a non-oxidizable Leu showed a great improvement. Furthermore, after treatment at 1 mM H2O2 where the wild-type is completely inactivated, full activity can be retained by engineering the Asn81, which might have conformational changes due to the environment of the pocket, to a non-bulky and non-oxidizable Ser." @default.
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- W2010096286 date "2001-11-27" @default.
- W2010096286 modified "2023-09-26" @default.
- W2010096286 title "Engineering of the H<sub>2</sub>O<sub>2</sub>-binding pocket region of a recombinant manganese peroxidase to be resistant to H<sub>2</sub>O<sub>2</sub>" @default.
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- W2010096286 doi "https://doi.org/10.1016/s0014-5793(01)03127-1" @default.
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