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• W2010115170 abstract "Abstract Objectives We were interested in determining whether epidermal growth factor gene‐transfected mesenchymal stem cells ( EGF ‐ MSC ) would accelerate fibroblast migration and proliferation. Materials and methods Fibroblasts were cultured in serum‐free conditioned media from EGF ‐ MSC ; RT ‐ PCR was performed to detect expression of EGF gene in EGF ‐ MSC s. EGF protein levels in cell culture supernatants from EGF ‐ MSC were assayed by ELISA and proliferation of EGF ‐ MSC ‐treated fibroblasts was performed using MTT assay. Effects of EGF ‐ MSC on fibroblast migration were evaluated using scratch wound and transmigration assays. Cell adhesion molecules, cell dynamics molecules and phospho‐(Ser) kinase substrate expressions of EGF ‐ MSC ‐treated fibroblasts were evaluated by western blotting. Results EGF gene expression increased in EGF ‐ MSC s and viability of EGF ‐ MSC ‐treated fibroblasts was elevated. EGF ‐ MSC ‐treated fibroblasts showed increased migration compared to controls. Expressions of cell adhesion molecules (β‐catenin, N‐cadherin), cell dynamics molecules (cofilin, ezrin) and phospho‐(Ser) kinase substrates (phospho‐MAPK/CDK substrate, phospho‐Arg‐(Ser)‐X‐Tyr/Phe‐X‐pSer motif) increased in EGF ‐ MSC ‐treated fibroblasts. These results imply that EGF ‐ MSC s contributed to enhancing the wound healing process by increased cell adhesion, dynamic effects, fibroblast migration, and proliferation. Conclusions This study indicates that EGF ‐ MSC s had a positive influence on fibroblast migration and proliferation and EGF ‐ MSC may provide a useful strategy for wound healing." @default.
• W2010115170 created "2016-06-24" @default.
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• W2010115170 date "2013-07-21" @default.
• W2010115170 modified "2023-09-27" @default.
• W2010115170 title "Effects of human epidermal growth factor gene‐transfected mesenchymal stem cells on fibroblast migration and proliferation" @default.
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• W2010115170 doi "https://doi.org/10.1111/cpr.12042" @default.
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