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- W2010126327 abstract "A study was undertaken to determine the subcellular location of DNase-1, a major acid deoxyribonuclease in Drosophila melanogaster. Embryonic tissue used in these experiments was derived from a wild type strain and from a strain homozygous at the DNase-1 locus (3–61.8) for the null activity allele, DNase-1n324. The majority of total acid DNase and DNase-1 activity is found in the small particulate fraction of tissue homogenates fractionated by differential centrifugation. The activity exhibits latency in these extracts indicating that it is membrane delimited. DNase-1 activity also co-equilibrates in sucrose density gradients with acid phosphatase activity which, in D. melanogaster, is known to be lysosomal. These results suggest that DNase-1 is localized largely within the lysosomes of embryonic tissue. Functional implications of a lysosomal location for DNase-1 are discussed." @default.
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- W2010126327 date "1980-01-01" @default.
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- W2010126327 title "A subcellular localization of the gene product of the DNase-1 locus in Drosophila melanogaster" @default.
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- W2010126327 doi "https://doi.org/10.1016/0020-1790(80)90020-7" @default.
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