Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010130488> ?p ?o ?g. }
- W2010130488 endingPage "81" @default.
- W2010130488 startingPage "65" @default.
- W2010130488 abstract "Rat heart mitochondria have been incubated with concentrations of pyruvate from 50 to 500 μm as substrate in the presence or absence of an optimal concentration of palmitoylcarnitine and with respiration limited by phosphate acceptor. The rate of pyruvate utilization has been determined and compared with the amount of active (dephosphorylated) pyruvate dehydrogenase measured in samples of mitochondria taken throughout the experiments and assayed under Vmax conditions. At a given pyruvate concentration, differences in pyruvate utilization as a proportion of the content of active pyruvate dehydrogenase are attributed to differences in feed-back inhibition and interpreted in terms of the ratios of mitochondrial NAD+NADH and CoA/acetyl-CoA. Under most conditions, differences in inhibition can be attributed to differences in the CoA/acetyl-CoA ratio. Feed-back inhibition is very pronounced in the presence of palmitoylcarnitine. These parameters are also examined in the presence of dichloroacetate, used to raise the steady-state levels of active pyruvate dehydrogenase in the absence of changing the pyruvate concentration, and in the presence of various ratios of carnitine/acetylcarnitine, which predominantly change the mitochondrial CoA/acetyl-CoA ratio. The latter experiment provides evidence that a decrease in mitochondrial NAD+NADH ratio from 3.5 to 2.2 essentially balances an increase in CoA/acetyl-CoA ratio from 0.67 to 12 in modulating enzyme interconversion, whereas the change in CoA/acetyl-CoA ratio is preponderant in effecting feed-back inhibition. Increasing the free Ca2+ concentration of incubation media from 10−7 to 10−6m using CaCl2-ethylene glycol bis(β-aminoethyl ether)-N,N′-tetraacetic acid buffers is shown to increase the steady-state level of active pyruvate dehydrogenase in intact mitochondria, in the absence of added ionophores. Moreover, this activation is reversible. Effects of Ca2+ ions are dependent upon the poise of the enzyme interconversion system and were only seen in these experiments in the presence of palmitoylcarnitine." @default.
- W2010130488 created "2016-06-24" @default.
- W2010130488 creator A5012199473 @default.
- W2010130488 creator A5022729625 @default.
- W2010130488 date "1978-11-01" @default.
- W2010130488 modified "2023-10-15" @default.
- W2010130488 title "Relative importance of pyruvate dehydrogenase interconversion and feed-back inhibition in the effect of fatty acids on pyruvate oxidation by rat heart mitochondria" @default.
- W2010130488 cites W128646528 @default.
- W2010130488 cites W1484792585 @default.
- W2010130488 cites W1492682176 @default.
- W2010130488 cites W1510598783 @default.
- W2010130488 cites W1514690741 @default.
- W2010130488 cites W1541911552 @default.
- W2010130488 cites W1564309614 @default.
- W2010130488 cites W1574890413 @default.
- W2010130488 cites W1583094119 @default.
- W2010130488 cites W1597115599 @default.
- W2010130488 cites W1609008040 @default.
- W2010130488 cites W1759931938 @default.
- W2010130488 cites W181688216 @default.
- W2010130488 cites W183190858 @default.
- W2010130488 cites W1851479110 @default.
- W2010130488 cites W1865773512 @default.
- W2010130488 cites W1966992999 @default.
- W2010130488 cites W1969195108 @default.
- W2010130488 cites W1977664507 @default.
- W2010130488 cites W1978885288 @default.
- W2010130488 cites W1986273448 @default.
- W2010130488 cites W1999793645 @default.
- W2010130488 cites W2006375605 @default.
- W2010130488 cites W2019113146 @default.
- W2010130488 cites W2020203890 @default.
- W2010130488 cites W2027603335 @default.
- W2010130488 cites W2032786413 @default.
- W2010130488 cites W2033517390 @default.
- W2010130488 cites W2034015091 @default.
- W2010130488 cites W2048455422 @default.
- W2010130488 cites W2052783498 @default.
- W2010130488 cites W2110014705 @default.
- W2010130488 cites W2115708290 @default.
- W2010130488 cites W2174520547 @default.
- W2010130488 cites W2214334965 @default.
- W2010130488 cites W2318787732 @default.
- W2010130488 cites W2319953554 @default.
- W2010130488 cites W2415721714 @default.
- W2010130488 cites W2469792820 @default.
- W2010130488 cites W50605420 @default.
- W2010130488 doi "https://doi.org/10.1016/0003-9861(78)90068-1" @default.
- W2010130488 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/216317" @default.
- W2010130488 hasPublicationYear "1978" @default.
- W2010130488 type Work @default.
- W2010130488 sameAs 2010130488 @default.
- W2010130488 citedByCount "128" @default.
- W2010130488 countsByYear W20101304882012 @default.
- W2010130488 countsByYear W20101304882013 @default.
- W2010130488 countsByYear W20101304882014 @default.
- W2010130488 countsByYear W20101304882015 @default.
- W2010130488 countsByYear W20101304882016 @default.
- W2010130488 countsByYear W20101304882017 @default.
- W2010130488 countsByYear W20101304882018 @default.
- W2010130488 countsByYear W20101304882020 @default.
- W2010130488 countsByYear W20101304882021 @default.
- W2010130488 crossrefType "journal-article" @default.
- W2010130488 hasAuthorship W2010130488A5012199473 @default.
- W2010130488 hasAuthorship W2010130488A5022729625 @default.
- W2010130488 hasConcept C101972281 @default.
- W2010130488 hasConcept C126093377 @default.
- W2010130488 hasConcept C140137476 @default.
- W2010130488 hasConcept C165135838 @default.
- W2010130488 hasConcept C181199279 @default.
- W2010130488 hasConcept C185592680 @default.
- W2010130488 hasConcept C190098323 @default.
- W2010130488 hasConcept C2776317432 @default.
- W2010130488 hasConcept C2777553358 @default.
- W2010130488 hasConcept C2777615046 @default.
- W2010130488 hasConcept C2778435403 @default.
- W2010130488 hasConcept C2778470496 @default.
- W2010130488 hasConcept C28859421 @default.
- W2010130488 hasConcept C55493867 @default.
- W2010130488 hasConcept C62231903 @default.
- W2010130488 hasConcept C75520062 @default.
- W2010130488 hasConcept C80097345 @default.
- W2010130488 hasConcept C88099638 @default.
- W2010130488 hasConcept C9506865 @default.
- W2010130488 hasConceptScore W2010130488C101972281 @default.
- W2010130488 hasConceptScore W2010130488C126093377 @default.
- W2010130488 hasConceptScore W2010130488C140137476 @default.
- W2010130488 hasConceptScore W2010130488C165135838 @default.
- W2010130488 hasConceptScore W2010130488C181199279 @default.
- W2010130488 hasConceptScore W2010130488C185592680 @default.
- W2010130488 hasConceptScore W2010130488C190098323 @default.
- W2010130488 hasConceptScore W2010130488C2776317432 @default.
- W2010130488 hasConceptScore W2010130488C2777553358 @default.
- W2010130488 hasConceptScore W2010130488C2777615046 @default.
- W2010130488 hasConceptScore W2010130488C2778435403 @default.
- W2010130488 hasConceptScore W2010130488C2778470496 @default.
- W2010130488 hasConceptScore W2010130488C28859421 @default.
- W2010130488 hasConceptScore W2010130488C55493867 @default.