FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010147335> ?p ?o ?g. }

• W2010147335 endingPage "889" @default.
• W2010147335 startingPage "880" @default.
• W2010147335 abstract "High-level expression of a recombinant protein in Chinese hamster ovary (CHO) cells typically requires the laborious and time-consuming procedure of stepwise gene amplification. We hypothesized that use of transcription control regions from a highly expressed gene in CHO cells to drive expression of a gene of interest might reduce the requirement for gene amplification. To this end, we cloned a 19 kb DNA fragment containing the Chinese hamster elongation factor-1alpha (EF-1alpha) gene, as well as 12 kb of 5' flanking sequence and 4 kb of 3' flanking sequence. Expression vectors containing 5' and 3' flanking sequences from the Chinese hamster EF-1alpha (CHEF1) gene were constructed and, after insertion of six different reporter genes, transfected into CHO cells. For comparison, CHO cells were also transfected with the same six reporter genes inserted into commercial vectors utilizing either the immediate early promoter from cytomegalovirus (CMV) or the human EF-1alpha promoter. The striking result from these studies was that average expression levels from pooled, stable transfectants of CHEF1 vectors were 6- to 35-fold higher than expression levels from commercial vectors that utilize the CMV or the human EF-1alpha promoters. We also used a CHEF1 vector to express a secreted and a membrane-bound protein in stably transfected non-CHO cell lines. CHEF1-driven expression of secreted alkaline phosphatase (SEAP) in three of four cell lines tested (HEK 293, K562, L1.2, and HCT 116) was 13- to 280-fold greater than that from a commercial vector employing the CMV promoter. After transfection of four different cell lines of hematopoietic origin (K562, L1.2, JY, and Jurkat), the CHEF1 vector was found to express the chemokine receptor CCR4 at >10-fold higher levels than that driven from a commercial vector utilizing the CMV promoter. Results from these experiments suggest that the CHEF1 vectors will be useful for high-level protein expression not only in CHO cells, but also in a variety of other mammalian cell lines." @default.
• W2010147335 created "2016-06-24" @default.
• W2010147335 creator A5062652111 @default.
• W2010147335 creator A5069386530 @default.
• W2010147335 date "2004-06-04" @default.
• W2010147335 modified "2023-10-17" @default.
• W2010147335 title "High-Level Expression of Proteins in Mammalian Cells Using Transcription Regulatory Sequences from the Chinese Hamster EF-1α Gene" @default.
• W2010147335 cites W1495020242 @default.
• W2010147335 cites W1716807955 @default.
• W2010147335 cites W1953331331 @default.
• W2010147335 cites W1969770714 @default.
• W2010147335 cites W1995391727 @default.
• W2010147335 cites W1997422287 @default.
• W2010147335 cites W1997541508 @default.
• W2010147335 cites W2002029926 @default.
• W2010147335 cites W2008895292 @default.
• W2010147335 cites W2022297396 @default.
• W2010147335 cites W2045348184 @default.
• W2010147335 cites W2049610944 @default.
• W2010147335 cites W2078618801 @default.
• W2010147335 cites W2084324934 @default.
• W2010147335 cites W2090727689 @default.
• W2010147335 cites W2095531021 @default.
• W2010147335 cites W2110182011 @default.
• W2010147335 cites W2113446079 @default.
• W2010147335 cites W2118781875 @default.
• W2010147335 cites W2142184151 @default.
• W2010147335 cites W2144456776 @default.
• W2010147335 cites W2148902400 @default.
• W2010147335 cites W2154685907 @default.
• W2010147335 cites W2169514240 @default.
• W2010147335 cites W91986915 @default.
• W2010147335 doi "https://doi.org/10.1021/bp034383r" @default.
• W2010147335 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15176895" @default.
• W2010147335 hasPublicationYear "2004" @default.
• W2010147335 type Work @default.
• W2010147335 sameAs 2010147335 @default.
• W2010147335 citedByCount "116" @default.
• W2010147335 countsByYear W20101473352012 @default.
• W2010147335 countsByYear W20101473352013 @default.
• W2010147335 countsByYear W20101473352014 @default.
• W2010147335 countsByYear W20101473352015 @default.
• W2010147335 countsByYear W20101473352016 @default.
• W2010147335 countsByYear W20101473352017 @default.
• W2010147335 countsByYear W20101473352018 @default.
• W2010147335 countsByYear W20101473352019 @default.
• W2010147335 countsByYear W20101473352020 @default.
• W2010147335 countsByYear W20101473352021 @default.
• W2010147335 countsByYear W20101473352022 @default.
• W2010147335 countsByYear W20101473352023 @default.
• W2010147335 crossrefType "journal-article" @default.
• W2010147335 hasAuthorship W2010147335A5062652111 @default.
• W2010147335 hasAuthorship W2010147335A5069386530 @default.
• W2010147335 hasConcept C101762097 @default.
• W2010147335 hasConcept C104317684 @default.
• W2010147335 hasConcept C138885662 @default.
• W2010147335 hasConcept C150194340 @default.
• W2010147335 hasConcept C153911025 @default.
• W2010147335 hasConcept C161733203 @default.
• W2010147335 hasConcept C174510640 @default.
• W2010147335 hasConcept C175656101 @default.
• W2010147335 hasConcept C179926584 @default.
• W2010147335 hasConcept C33161422 @default.
• W2010147335 hasConcept C40767141 @default.
• W2010147335 hasConcept C41895202 @default.
• W2010147335 hasConcept C54009773 @default.
• W2010147335 hasConcept C54355233 @default.
• W2010147335 hasConcept C81885089 @default.
• W2010147335 hasConcept C86803240 @default.
• W2010147335 hasConceptScore W2010147335C101762097 @default.
• W2010147335 hasConceptScore W2010147335C104317684 @default.
• W2010147335 hasConceptScore W2010147335C138885662 @default.
• W2010147335 hasConceptScore W2010147335C150194340 @default.
• W2010147335 hasConceptScore W2010147335C153911025 @default.
• W2010147335 hasConceptScore W2010147335C161733203 @default.
• W2010147335 hasConceptScore W2010147335C174510640 @default.
• W2010147335 hasConceptScore W2010147335C175656101 @default.
• W2010147335 hasConceptScore W2010147335C179926584 @default.
• W2010147335 hasConceptScore W2010147335C33161422 @default.
• W2010147335 hasConceptScore W2010147335C40767141 @default.
• W2010147335 hasConceptScore W2010147335C41895202 @default.
• W2010147335 hasConceptScore W2010147335C54009773 @default.
• W2010147335 hasConceptScore W2010147335C54355233 @default.
• W2010147335 hasConceptScore W2010147335C81885089 @default.
• W2010147335 hasConceptScore W2010147335C86803240 @default.
• W2010147335 hasIssue "3" @default.
• W2010147335 hasLocation W20101473351 @default.
• W2010147335 hasLocation W20101473352 @default.
• W2010147335 hasOpenAccess W2010147335 @default.
• W2010147335 hasPrimaryLocation W20101473351 @default.
• W2010147335 hasRelatedWork W1965269756 @default.
• W2010147335 hasRelatedWork W2053393868 @default.
• W2010147335 hasRelatedWork W2359175313 @default.
• W2010147335 hasRelatedWork W2359813594 @default.
• W2010147335 hasRelatedWork W2363386379 @default.
• W2010147335 hasRelatedWork W2376295338 @default.
• W2010147335 hasRelatedWork W2392112009 @default.
• W2010147335 hasRelatedWork W2392841809 @default.

• next