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- W2010167991 abstract "Haploid plants of Nicotiana tabacum were obtained by culturing anthers at the microspore stage. Explants of pith tissue from haploid as well as diploid plants were grown in vitro, and changes in cell number, cell size, and fresh weight were determined over a 16-day period. In the tissue from diploid plants a decrease in cell size was seen after the eighth day in culture, but the tissues from haploid plants exhibited a slight increase in cell size. Spectrophotometric determination of deoxyribonucleic acid (DNA) was made to investigate the time course of DNA synthesis during cell proliferation and culture. A wide range of ploidy levels was seen in the fresh pith tissues of both haploid and diploid .plants. Culture of these tissues resulted in increased levels of ploidy in a large proportion of the cells. In general, cells with lower DNA content per nucleus entered the cell cycle sooner than those with a higher amount. After an extended period of time in culture both haploid and diploid cxplants showed a variety of polyploid and aneuploid cells. The effects of pfluorophenylalanine (PFP) on explant growth and ploidy were tested. PFP was inhibitory to the growth of all cells, but haploid cells were inhibited slightly less than those of higher ploidy. Spectrophotometric data are presented to demonstrate that PFP can be used to maintain - or even increase - the population of haploid cells in tissue cultures derived from haploid plants of tobacco." @default.
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- W2010167991 date "1976-01-01" @default.
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- W2010167991 title "The Dynamics of Cell Proliferation in Haploid and Diploid Tissues of Nicotiana tabacum" @default.
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- W2010167991 doi "https://doi.org/10.1016/s0044-328x(76)80199-7" @default.
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