FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010181565> ?p ?o ?g. }

• W2010181565 endingPage "e114386" @default.
• W2010181565 startingPage "e114386" @default.
• W2010181565 abstract "To investigate the efficacy and mechanism of tacrolimus(FK506), which is a novel macrolide immunosuppressant, in inhibiting triggering receptor expressed on myeloid cells-1 (TREM-1) expression in a murine keratitis model induced by Aspergillus fumigatus.TREM-1 was detected in 11 fungus-infected human corneas by quantitative real-time PCR (qRT-PCR). RAW264.7 macrophages were divided into four groups, which received treatment with zymosan (100 µg/ml), zymosan (100 µg/ml) + mTREM-1/Fc protein (1 µg/ml), or zymosan (100 µg/ml) + FK506 (20 µM) or negative-control treatment. After this treatment, the expression of TREM-1, interleukin-1β (IL-1β) and tumor necrosis factor α (TNFα) was assayed using qRT-PCR and ELISA. The mouse model of fungal keratitis was created by intrastromal injection with Aspergillus fumigatus, and the mice were divided into 2 groups: group A received vehicle eye drops 4 times each day, and group B received 4 doses of FK506 eye drops each day. Corneal damage was evaluated by clinical scoring and histologic examination,and myeloperoxidase (MPO) protein levels were also detected by ELISA. The expression of TREM-1, IL-1β and TNFα was then determined at different time points using qRT-PCR and ELISA.TREM-1 expression dramatically increased in the human corneas with fungal keratitis. In contrast, FK506 reduced the expression of TREM-1, IL-1β and TNFα in RAW264.7 macrophages stimulated with zymosan. In the mouse model, at day 1 post-infection, the corneal score of the FK506-treated group was lower than that of the control, and polymorphonuclear neutrophil (PMN) infiltration was diminished. TREM-1, IL-1β and TNFα expression was significantly reduced at the same time point. However, the statistically significant differences in cytokine expression, clinical scores and infiltration disappeared at 5 days post-infection.FK506 may inhibit the inflammation induced by fungi and alleviate the severity of corneal damage at an early stage of fungal keratitis by downregulating TREM-1 expression." @default.
• W2010181565 created "2016-06-24" @default.
• W2010181565 creator A5002069152 @default.
• W2010181565 creator A5006337727 @default.
• W2010181565 creator A5007568711 @default.
• W2010181565 creator A5044887331 @default.
• W2010181565 creator A5050134485 @default.
• W2010181565 creator A5054347574 @default.
• W2010181565 creator A5056695279 @default.
• W2010181565 creator A5059282344 @default.
• W2010181565 creator A5076596955 @default.
• W2010181565 creator A5087787304 @default.
• W2010181565 date "2014-12-02" @default.
• W2010181565 modified "2023-10-11" @default.
• W2010181565 title "Tacrolimus (FK506) Suppresses TREM-1 Expression at an Early but Not at a Late Stage in a Murine Model of Fungal Keratitis" @default.
• W2010181565 cites W1539977736 @default.
• W2010181565 cites W1561206187 @default.
• W2010181565 cites W1575122600 @default.
• W2010181565 cites W1601476460 @default.
• W2010181565 cites W162447880 @default.
• W2010181565 cites W1643888758 @default.
• W2010181565 cites W171604402 @default.
• W2010181565 cites W1771141216 @default.
• W2010181565 cites W1968626772 @default.
• W2010181565 cites W1974650056 @default.
• W2010181565 cites W1979947873 @default.
• W2010181565 cites W1983148285 @default.
• W2010181565 cites W1983462777 @default.
• W2010181565 cites W1984947099 @default.
• W2010181565 cites W1986899130 @default.
• W2010181565 cites W1991385417 @default.
• W2010181565 cites W1991999800 @default.
• W2010181565 cites W1994243439 @default.
• W2010181565 cites W2008199540 @default.
• W2010181565 cites W2008859777 @default.
• W2010181565 cites W2020360260 @default.
• W2010181565 cites W2020370872 @default.
• W2010181565 cites W2025130647 @default.
• W2010181565 cites W2025918211 @default.
• W2010181565 cites W2038075308 @default.
• W2010181565 cites W2040957577 @default.
• W2010181565 cites W2045585355 @default.
• W2010181565 cites W2045996807 @default.
• W2010181565 cites W2047796994 @default.
• W2010181565 cites W2047855993 @default.
• W2010181565 cites W2060727135 @default.
• W2010181565 cites W2071110455 @default.
• W2010181565 cites W2074857991 @default.
• W2010181565 cites W2092597654 @default.
• W2010181565 cites W2098805814 @default.
• W2010181565 cites W2101316389 @default.
• W2010181565 cites W2106141541 @default.
• W2010181565 cites W2116546952 @default.
• W2010181565 cites W2122104511 @default.
• W2010181565 cites W2126924404 @default.
• W2010181565 cites W2128413447 @default.
• W2010181565 cites W2135414937 @default.
• W2010181565 cites W2137621165 @default.
• W2010181565 cites W2139106536 @default.
• W2010181565 cites W2147224863 @default.
• W2010181565 cites W2149698238 @default.
• W2010181565 cites W2153077179 @default.
• W2010181565 cites W2155533398 @default.
• W2010181565 cites W2172069549 @default.
• W2010181565 cites W4256074825 @default.
• W2010181565 doi "https://doi.org/10.1371/journal.pone.0114386" @default.
• W2010181565 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4252117" @default.
• W2010181565 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25464008" @default.
• W2010181565 hasPublicationYear "2014" @default.
• W2010181565 type Work @default.
• W2010181565 sameAs 2010181565 @default.
• W2010181565 citedByCount "14" @default.
• W2010181565 countsByYear W20101815652016 @default.
• W2010181565 countsByYear W20101815652017 @default.
• W2010181565 countsByYear W20101815652018 @default.
• W2010181565 countsByYear W20101815652019 @default.
• W2010181565 countsByYear W20101815652020 @default.
• W2010181565 countsByYear W20101815652022 @default.
• W2010181565 crossrefType "journal-article" @default.
• W2010181565 hasAuthorship W2010181565A5002069152 @default.
• W2010181565 hasAuthorship W2010181565A5006337727 @default.
• W2010181565 hasAuthorship W2010181565A5007568711 @default.
• W2010181565 hasAuthorship W2010181565A5044887331 @default.
• W2010181565 hasAuthorship W2010181565A5050134485 @default.
• W2010181565 hasAuthorship W2010181565A5054347574 @default.
• W2010181565 hasAuthorship W2010181565A5056695279 @default.
• W2010181565 hasAuthorship W2010181565A5059282344 @default.
• W2010181565 hasAuthorship W2010181565A5076596955 @default.
• W2010181565 hasAuthorship W2010181565A5087787304 @default.
• W2010181565 hasBestOaLocation W20101815651 @default.
• W2010181565 hasConcept C104317684 @default.
• W2010181565 hasConcept C118487528 @default.
• W2010181565 hasConcept C16685009 @default.
• W2010181565 hasConcept C17991360 @default.
• W2010181565 hasConcept C202751555 @default.
• W2010181565 hasConcept C203014093 @default.
• W2010181565 hasConcept C203565725 @default.
• W2010181565 hasConcept C2776079991 @default.

• next