Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010182589> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2010182589 endingPage "816" @default.
- W2010182589 startingPage "811" @default.
- W2010182589 abstract "A simple and effective salting-out method was developed for the purification of the metallo-β-lactamase CcrA from Bacteroides fragilis based on the plasmid pMSZ02, in which the crude protein secreted into growth medium was precipitated by 80% sulfate saturation of the medium and purified with Q-Sepharose to offer pure CcrA with yield of 20.1 mg per litter medium. The dependence of the amount of protein precipitation on sulfate saturation was investigated, which showed that more than 80% sulfate saturation resulted the maximum protein precipitated. The purified CcrA was evaluated by steady-state kinetics using penicillin G and cephalothin V as substrates, which showed the Km values of 68±2 and 17±2 µM and Kcat values of 63±1 and 102±3 S-1, respectively. The comparison with the data of the protein from literature method showed that the salting-out method was viable, and it could be useful for the purification of other proteins secreted into growth medium." @default.
- W2010182589 created "2016-06-24" @default.
- W2010182589 creator A5038390875 @default.
- W2010182589 creator A5057286088 @default.
- W2010182589 creator A5071335452 @default.
- W2010182589 creator A5081140206 @default.
- W2010182589 date "2013-10-01" @default.
- W2010182589 modified "2023-09-27" @default.
- W2010182589 title "Purification of metallo-B-lactamase CcrA from Bacteroides fragilis with salting-out method" @default.
- W2010182589 cites W1976915850 @default.
- W2010182589 cites W1983305461 @default.
- W2010182589 cites W1990071271 @default.
- W2010182589 cites W1996356495 @default.
- W2010182589 cites W2016668411 @default.
- W2010182589 cites W2024807799 @default.
- W2010182589 cites W2043516822 @default.
- W2010182589 cites W2055543222 @default.
- W2010182589 cites W2065668262 @default.
- W2010182589 cites W2079698340 @default.
- W2010182589 cites W2090133370 @default.
- W2010182589 cites W2098423864 @default.
- W2010182589 cites W2107326708 @default.
- W2010182589 cites W2119150772 @default.
- W2010182589 cites W2134352676 @default.
- W2010182589 cites W2143874510 @default.
- W2010182589 cites W2292239206 @default.
- W2010182589 cites W2395006014 @default.
- W2010182589 doi "https://doi.org/10.1590/s1516-89132013000500012" @default.
- W2010182589 hasPublicationYear "2013" @default.
- W2010182589 type Work @default.
- W2010182589 sameAs 2010182589 @default.
- W2010182589 citedByCount "0" @default.
- W2010182589 crossrefType "journal-article" @default.
- W2010182589 hasAuthorship W2010182589A5038390875 @default.
- W2010182589 hasAuthorship W2010182589A5057286088 @default.
- W2010182589 hasAuthorship W2010182589A5071335452 @default.
- W2010182589 hasAuthorship W2010182589A5081140206 @default.
- W2010182589 hasBestOaLocation W20101825891 @default.
- W2010182589 hasConcept C114614502 @default.
- W2010182589 hasConcept C121332964 @default.
- W2010182589 hasConcept C134121241 @default.
- W2010182589 hasConcept C148898269 @default.
- W2010182589 hasConcept C169490005 @default.
- W2010182589 hasConcept C178790620 @default.
- W2010182589 hasConcept C184651966 @default.
- W2010182589 hasConcept C185592680 @default.
- W2010182589 hasConcept C191897082 @default.
- W2010182589 hasConcept C192562407 @default.
- W2010182589 hasConcept C2780246119 @default.
- W2010182589 hasConcept C33923547 @default.
- W2010182589 hasConcept C43617362 @default.
- W2010182589 hasConcept C501593827 @default.
- W2010182589 hasConcept C55493867 @default.
- W2010182589 hasConcept C62520636 @default.
- W2010182589 hasConcept C9930424 @default.
- W2010182589 hasConceptScore W2010182589C114614502 @default.
- W2010182589 hasConceptScore W2010182589C121332964 @default.
- W2010182589 hasConceptScore W2010182589C134121241 @default.
- W2010182589 hasConceptScore W2010182589C148898269 @default.
- W2010182589 hasConceptScore W2010182589C169490005 @default.
- W2010182589 hasConceptScore W2010182589C178790620 @default.
- W2010182589 hasConceptScore W2010182589C184651966 @default.
- W2010182589 hasConceptScore W2010182589C185592680 @default.
- W2010182589 hasConceptScore W2010182589C191897082 @default.
- W2010182589 hasConceptScore W2010182589C192562407 @default.
- W2010182589 hasConceptScore W2010182589C2780246119 @default.
- W2010182589 hasConceptScore W2010182589C33923547 @default.
- W2010182589 hasConceptScore W2010182589C43617362 @default.
- W2010182589 hasConceptScore W2010182589C501593827 @default.
- W2010182589 hasConceptScore W2010182589C55493867 @default.
- W2010182589 hasConceptScore W2010182589C62520636 @default.
- W2010182589 hasConceptScore W2010182589C9930424 @default.
- W2010182589 hasIssue "5" @default.
- W2010182589 hasLocation W20101825891 @default.
- W2010182589 hasLocation W20101825892 @default.
- W2010182589 hasLocation W20101825893 @default.
- W2010182589 hasOpenAccess W2010182589 @default.
- W2010182589 hasPrimaryLocation W20101825891 @default.
- W2010182589 hasRelatedWork W1986991025 @default.
- W2010182589 hasRelatedWork W2062749443 @default.
- W2010182589 hasRelatedWork W2151422433 @default.
- W2010182589 hasRelatedWork W2401463829 @default.
- W2010182589 hasRelatedWork W2403975342 @default.
- W2010182589 hasRelatedWork W2949184651 @default.
- W2010182589 hasRelatedWork W2950122242 @default.
- W2010182589 hasRelatedWork W2950230984 @default.
- W2010182589 hasRelatedWork W2950847194 @default.
- W2010182589 hasRelatedWork W2952649848 @default.
- W2010182589 hasVolume "56" @default.
- W2010182589 isParatext "false" @default.
- W2010182589 isRetracted "false" @default.
- W2010182589 magId "2010182589" @default.
- W2010182589 workType "article" @default.