FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010222015> ?p ?o ?g. }

• W2010222015 endingPage "1759" @default.
• W2010222015 startingPage "1759" @default.
• W2010222015 abstract "Formulation, characterization, and expression of a recombinant MOMP Chlamydia trachomatis DNA vaccine encapsulated in chitosan nanoparticles Chino D Cambridge, Shree R Singh, Alain B Waffo, Stacie J Fairley, Vida A DennisCenter for NanoBiotechnology Research (CNBR), Alabama State University, Montgomery, AL, USAAbstract: Chlamydia trachomatis is a bacterial sexually transmitted infection affecting millions of people worldwide. Previous vaccination attempts have employed the recombinant major outer membrane protein (MOMP) of C. trachomatis nonetheless, with limited success, perhaps, due to stability, degradation, and delivery issues. In this study we cloned C. trachomatis recombinant MOMP DNA (DMOMP) and encapsulated it in chitosan nanoparticles (DMCNP) using the complex coacervation technique. Physiochemical characterizations of DMCNP included transmission and scanning electron microcopy, Fourier transform infrared and ultraviolet-visible spectroscopy, and zeta potential. Encapsulated DMOMP was 167–250 nm, with a uniform spherical shape and homogenous morphology, and an encapsulation efficiency > 90%. A slow release pattern of encapsulated DMOMP, especially in acidic solution, was observed over 7 days. The zeta potential of DMCNP was ~8.80 mV, which indicated that it was highly stable. Toxicity studies of DMCNP (25–400 µg/mL) to Cos-7 cells using the MTT assay revealed minimal toxicity over 24–72 hours with >90% viable cells. Ultra-violet visible (UV-vis) spectra indicated encapsulated DMOMP protection by chitosan, whereas agarose gel electrophoresis verified its protection from enzymatic degradation. Expression of MOMP protein in DMCNP-transfected Cos-7 cells was demonstrated via Western blotting and immunofluorescence microscopy. Significantly, intramuscular injection of BALB/c mice with DMCNP confirmed the delivery of encapsulated DMOMP, and expression of the MOMP gene transcript in thigh muscles and spleens. Our data show that encapsulation of DMOMP in biodegradable chitosan nanoparticles imparts stability and protection from enzymatic digestion, and enhances delivery and expression of DMOMP in vitro and in mice. Further investigations of the nanoencapsulated DMCNP vaccine formulation against C. trachomatis in mice are warranted.Keywords: Chlamydia trachomatis, bacteria, chitosan, DNA, MOMP, vaccine" @default.
• W2010222015 created "2016-06-24" @default.
• W2010222015 creator A5011502095 @default.
• W2010222015 creator A5014268356 @default.
• W2010222015 creator A5044283007 @default.
• W2010222015 creator A5059834789 @default.
• W2010222015 creator A5063851790 @default.
• W2010222015 date "2013-05-01" @default.
• W2010222015 modified "2023-10-14" @default.
• W2010222015 title "Formulation, characterization, and expression of a recombinant MOMP Chlamydia trachomatis DNA vaccine encapsulated in chitosan nanoparticles" @default.
• W2010222015 cites W1498725963 @default.
• W2010222015 cites W1850492787 @default.
• W2010222015 cites W1971652191 @default.
• W2010222015 cites W1978643345 @default.
• W2010222015 cites W1982096781 @default.
• W2010222015 cites W1982593016 @default.
• W2010222015 cites W1992287346 @default.
• W2010222015 cites W1993655662 @default.
• W2010222015 cites W1998036925 @default.
• W2010222015 cites W2008039399 @default.
• W2010222015 cites W2009741912 @default.
• W2010222015 cites W2010860761 @default.
• W2010222015 cites W2013998409 @default.
• W2010222015 cites W2014178660 @default.
• W2010222015 cites W2022922808 @default.
• W2010222015 cites W2029392498 @default.
• W2010222015 cites W2033244141 @default.
• W2010222015 cites W2033530261 @default.
• W2010222015 cites W2034783507 @default.
• W2010222015 cites W2037969693 @default.
• W2010222015 cites W2042053017 @default.
• W2010222015 cites W2043510152 @default.
• W2010222015 cites W2044486914 @default.
• W2010222015 cites W2047798559 @default.
• W2010222015 cites W2048546608 @default.
• W2010222015 cites W2049683154 @default.
• W2010222015 cites W2050887188 @default.
• W2010222015 cites W2057144911 @default.
• W2010222015 cites W2076224340 @default.
• W2010222015 cites W2076498486 @default.
• W2010222015 cites W2082641947 @default.
• W2010222015 cites W2087313309 @default.
• W2010222015 cites W2089773350 @default.
• W2010222015 cites W2092967539 @default.
• W2010222015 cites W2095507086 @default.
• W2010222015 cites W2125255269 @default.
• W2010222015 cites W2127415665 @default.
• W2010222015 cites W2127487757 @default.
• W2010222015 cites W2134618540 @default.
• W2010222015 cites W2139501544 @default.
• W2010222015 cites W2146129300 @default.
• W2010222015 cites W2166325259 @default.
• W2010222015 cites W2168359 @default.
• W2010222015 cites W2168591865 @default.
• W2010222015 cites W2411038802 @default.
• W2010222015 cites W2762983275 @default.
• W2010222015 cites W970796 @default.
• W2010222015 cites W2376888879 @default.
• W2010222015 doi "https://doi.org/10.2147/ijn.s42723" @default.
• W2010222015 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3656902" @default.
• W2010222015 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23690681" @default.
• W2010222015 hasPublicationYear "2013" @default.
• W2010222015 type Work @default.
• W2010222015 sameAs 2010222015 @default.
• W2010222015 citedByCount "20" @default.
• W2010222015 countsByYear W20102220152014 @default.
• W2010222015 countsByYear W20102220152015 @default.
• W2010222015 countsByYear W20102220152016 @default.
• W2010222015 countsByYear W20102220152017 @default.
• W2010222015 countsByYear W20102220152018 @default.
• W2010222015 countsByYear W20102220152019 @default.
• W2010222015 countsByYear W20102220152020 @default.
• W2010222015 countsByYear W20102220152021 @default.
• W2010222015 countsByYear W20102220152022 @default.
• W2010222015 countsByYear W20102220152023 @default.
• W2010222015 crossrefType "journal-article" @default.
• W2010222015 hasAuthorship W2010222015A5011502095 @default.
• W2010222015 hasAuthorship W2010222015A5014268356 @default.
• W2010222015 hasAuthorship W2010222015A5044283007 @default.
• W2010222015 hasAuthorship W2010222015A5059834789 @default.
• W2010222015 hasAuthorship W2010222015A5063851790 @default.
• W2010222015 hasBestOaLocation W20102220151 @default.
• W2010222015 hasConcept C104317684 @default.
• W2010222015 hasConcept C13965031 @default.
• W2010222015 hasConcept C153911025 @default.
• W2010222015 hasConcept C155672457 @default.
• W2010222015 hasConcept C159047783 @default.
• W2010222015 hasConcept C167508917 @default.
• W2010222015 hasConcept C171250308 @default.
• W2010222015 hasConcept C185592680 @default.
• W2010222015 hasConcept C192562407 @default.
• W2010222015 hasConcept C2777391075 @default.
• W2010222015 hasConcept C2779732960 @default.
• W2010222015 hasConcept C40767141 @default.
• W2010222015 hasConcept C55493867 @default.
• W2010222015 hasConcept C86181022 @default.
• W2010222015 hasConcept C86803240 @default.
• W2010222015 hasConcept C89423630 @default.

• next