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- W2010248842 abstract "Abstract A simple, rapid procedure for quantitative estimation of phenylthiohydantoins liberated in the Edman degradation is described. Peptides are degraded using 3H-phenylisothiocyanate. The radioactive thiazolinones released in the acid cleavage step of the degradation are added to a mixture of unlabelled phenylthiobydantoins and are isomerized in situ to the corresponding phenylthiohydaatoins. The phenylthiobydantoin mixture is separated using thin layer chromatography, ultraviolet-absorbing areas are scraped off, and radioactive areas are located by liquid scintillation counting. This procedure is rapid, sensitive and inexpensive. No specialized apparatus other than a scintillation counter is required. The procedure is especially applicable to automated versions of the Edman degradation." @default.
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- W2010248842 date "1969-11-01" @default.
- W2010248842 modified "2023-10-15" @default.
- W2010248842 title "An isotope dilution procedure for detection and quantitative estimation of phenylthiohydantoins released in the Edman degradation" @default.
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- W2010248842 doi "https://doi.org/10.1016/0006-291x(69)90862-6" @default.
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