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- W2010261476 abstract "Both the miR-29 family and TGF-ß have been shown to play a role in pathologic fibrosis. The goal of this study is to understand the role of TGF-ß and miR-29 in the pathogenesis of leiomyoma. Basic Science - Experimental. Tissue was obtained from women undergoing hysterectomy (n=18). MiR-29 expression in vivo was determined with PCR. Primary leiomyoma and myometrial cells were isolated from the tissue and cultured. MiR-29 expression was manipulated in vitro. Dose response (0-10 ng/ml) and time response (0-120 min) treatments were performed on the cells with TGF-ß. Cells were also treated with TGF-ß for 3 days. RNA was isolated and PCR was performed for collagen and miR-29. Cell protein was isolated and Western blots were performed for SMAD2 and SMAD3. Luciferase assays containing the COL1A1 3'UTR were performed inTGF-ß versus vehicle treated cells. All species of miR-29 expression (a,b,and c) were decreased in leiomyoma versus matched myometrium (P<0.01). Overexpression of miR-29 lead to decreased collagen expression. SMAD2 was shown to increase in TGF-ß treated leiomyoma cells in both a dose and time dependent fashion but not in myometrium. TGF-ß treatment (10 ng/ml) resulted in increased collagen expression in leiomyoma cells but not myometrial cells as well as decreased expression of miR-29 versus vehicle (P=0.02). Luciferase expression was increased in cells treated with TGF-ß versus vehicle (P=0.03). We have demonstrated that miR-29 is differentially expressed in leiomyoma versus myometrium and plays a role in the differential expression of the major fibrillar collagens seen in these two tissues. Further, miR-29 is downregulated by TGF-ß, a growth factor known to be overexpressed in leiomyoma versus myometrium. Downregulated miR-29 leads to increased expression of the major fibrillar collagens and contributes to the excessive extracelluar matrix seen in leiomyoma versus myometrium." @default.
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- W2010261476 date "2012-09-01" @default.
- W2010261476 modified "2023-10-14" @default.
- W2010261476 title "Transforming growth factor beta upregulates collagen via the suppression of microrna-29 expression in leiomyoma" @default.
- W2010261476 doi "https://doi.org/10.1016/j.fertnstert.2012.07.258" @default.
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