FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010289929> ?p ?o ?g. }

• W2010289929 endingPage "484" @default.
• W2010289929 startingPage "476" @default.
• W2010289929 abstract "Starch and glycogen are widespread storage polysaccharides in bacteria, plants, and animals. Recently, some cyanobacteria were found to accumulate water-insoluble α-glucan similar to amylopectin rather than glycogen, the latter of which is more commonly produced in these organisms. The amylopectin-producing species including Cyanobacterium sp. NBRC 102756 invariably have three branching enzyme (BE) homologs, BE1, BE2, and BE3, all belonging to the glycoside hydrolase family 13. Multiple BE isoforms in prokaryotes have not been previously studied. In the present work, we carried out functional characterization of these enzymes expressed in Escherichia coli. The recombinant enzymes were all active, although the specific activity of BE3 was much lower than those of BE1 and BE2. After the incubation of the enzymes with amylopectin or amylose, the reaction products were analyzed by fluorophore-assisted carbohydrate capillary electrophoresis method. BE1 and BE2 showed similar chain-length preference to BEIIb isoform of rice (Oryza sativa L.), while the catalytic specificity of BE3 was similar to that of rice BEI. These results indicate that starch-producing cyanobacteria have both type-I BE (BE3) and type-II BEs (BE1 and BE2) in terms of chain-length preferences, as is the case of plants. All BE isoforms were active against phosphorylase limit dextrin, in which outer branches had been uniformly diminished to 4 glucose residues. Based on its catalytic properties, BE3 was assumed to have a role to transfer the glucan chain bearing branch(es) to give rise to a newly growing unit, or cluster as observed in amylopectin molecule." @default.
• W2010289929 created "2016-06-24" @default.
• W2010289929 creator A5000647160 @default.
• W2010289929 creator A5025262975 @default.
• W2010289929 creator A5039665798 @default.
• W2010289929 creator A5055340299 @default.
• W2010289929 creator A5067466334 @default.
• W2010289929 creator A5067912734 @default.
• W2010289929 creator A5077310835 @default.
• W2010289929 creator A5082421988 @default.
• W2010289929 creator A5082851201 @default.
• W2010289929 creator A5086539409 @default.
• W2010289929 date "2015-05-01" @default.
• W2010289929 modified "2023-09-27" @default.
• W2010289929 title "Functional characterization of three (GH13) branching enzymes involved in cyanobacterial starch biosynthesis from Cyanobacterium sp. NBRC 102756" @default.
• W2010289929 cites W1703928197 @default.
• W2010289929 cites W1967992666 @default.
• W2010289929 cites W1968559207 @default.
• W2010289929 cites W1974343083 @default.
• W2010289929 cites W1975275461 @default.
• W2010289929 cites W1981779399 @default.
• W2010289929 cites W1985629904 @default.
• W2010289929 cites W1987773708 @default.
• W2010289929 cites W1990540495 @default.
• W2010289929 cites W1990740891 @default.
• W2010289929 cites W2007350561 @default.
• W2010289929 cites W2008362554 @default.
• W2010289929 cites W2019528226 @default.
• W2010289929 cites W2023363453 @default.
• W2010289929 cites W2031498839 @default.
• W2010289929 cites W2033988520 @default.
• W2010289929 cites W2038781049 @default.
• W2010289929 cites W2041100300 @default.
• W2010289929 cites W2059560880 @default.
• W2010289929 cites W2060101782 @default.
• W2010289929 cites W2062909887 @default.
• W2010289929 cites W2072810051 @default.
• W2010289929 cites W2074483275 @default.
• W2010289929 cites W2090080358 @default.
• W2010289929 cites W2090121310 @default.
• W2010289929 cites W2091966562 @default.
• W2010289929 cites W2099188280 @default.
• W2010289929 cites W2100920419 @default.
• W2010289929 cites W2108929776 @default.
• W2010289929 cites W2108976000 @default.
• W2010289929 cites W2109399911 @default.
• W2010289929 cites W2110149607 @default.
• W2010289929 cites W2110958200 @default.
• W2010289929 cites W2114874948 @default.
• W2010289929 cites W2115094171 @default.
• W2010289929 cites W2117329205 @default.
• W2010289929 cites W2122799947 @default.
• W2010289929 cites W2123887225 @default.
• W2010289929 cites W2124269299 @default.
• W2010289929 cites W2124346974 @default.
• W2010289929 cites W2125299168 @default.
• W2010289929 cites W2126441454 @default.
• W2010289929 cites W2130753366 @default.
• W2010289929 cites W2137015675 @default.
• W2010289929 cites W2139052360 @default.
• W2010289929 cites W2139166908 @default.
• W2010289929 cites W2139759180 @default.
• W2010289929 cites W2145673348 @default.
• W2010289929 cites W2159341724 @default.
• W2010289929 cites W2163367030 @default.
• W2010289929 cites W2167879487 @default.
• W2010289929 cites W4294309038 @default.
• W2010289929 doi "https://doi.org/10.1016/j.bbapap.2015.02.012" @default.
• W2010289929 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25731081" @default.
• W2010289929 hasPublicationYear "2015" @default.
• W2010289929 type Work @default.
• W2010289929 sameAs 2010289929 @default.
• W2010289929 citedByCount "20" @default.
• W2010289929 countsByYear W20102899292015 @default.
• W2010289929 countsByYear W20102899292016 @default.
• W2010289929 countsByYear W20102899292017 @default.
• W2010289929 countsByYear W20102899292018 @default.
• W2010289929 countsByYear W20102899292019 @default.
• W2010289929 countsByYear W20102899292020 @default.
• W2010289929 countsByYear W20102899292021 @default.
• W2010289929 countsByYear W20102899292022 @default.
• W2010289929 countsByYear W20102899292023 @default.
• W2010289929 crossrefType "journal-article" @default.
• W2010289929 hasAuthorship W2010289929A5000647160 @default.
• W2010289929 hasAuthorship W2010289929A5025262975 @default.
• W2010289929 hasAuthorship W2010289929A5039665798 @default.
• W2010289929 hasAuthorship W2010289929A5055340299 @default.
• W2010289929 hasAuthorship W2010289929A5067466334 @default.
• W2010289929 hasAuthorship W2010289929A5067912734 @default.
• W2010289929 hasAuthorship W2010289929A5077310835 @default.
• W2010289929 hasAuthorship W2010289929A5082421988 @default.
• W2010289929 hasAuthorship W2010289929A5082851201 @default.
• W2010289929 hasAuthorship W2010289929A5086539409 @default.
• W2010289929 hasConcept C100817775 @default.
• W2010289929 hasConcept C104317684 @default.
• W2010289929 hasConcept C150487720 @default.
• W2010289929 hasConcept C170835558 @default.
• W2010289929 hasConcept C181199279 @default.

• next