Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010334049> ?p ?o ?g. }
- W2010334049 endingPage "508" @default.
- W2010334049 startingPage "502" @default.
- W2010334049 abstract "Objectives Karyotyping on chorionic villous samples (CVS) includes the analysis of both cytotrophoblast (STC) and mesenchyme (LTC). This approach requires complex laboratory organization and trained technicians. The introduction of quantitative fluorescent polymerase chain reaction (QF-PCR) instead of conventional karyotyping in low-risk pregnancies opened its application in CVS analysis. Discordant QF-PCR and CVS cytogenetic results were reported, and strategies for CVS analysis were introduced to minimize this risk. The possibility to substitute the STC with QF-PCR was reported. The aim of this study is to evaluate benefits and limitations of the approach QF-PCR + LTC compared with the traditional method STC + LTC and to quantify the associated risks of false results. Method This study is based on a retrospective cytogenetic audit of CVS results (n = 44 727) generated by the STC + LTC analytic approach. False-negative risks related to true fetal mosaicism type IV, imprinting syndromes and maternal contamination in LTC were calculated. Results Compared with STC + LTC, QF-PCR + LTC approach is associated with a cumulative false-negative risk of ~1/3100–1/4400. Costs and reporting time of STC in a high-throughput cytogenetic lab are similar to a CE-IVD marked QF-PCR analysis. Conclusions These results should be clearly highlighted in the pre-test counseling and extensively discussed with the couple prior to testing for informed consent. © 2013 John Wiley & Sons, Ltd." @default.
- W2010334049 created "2016-06-24" @default.
- W2010334049 creator A5004851952 @default.
- W2010334049 creator A5010977968 @default.
- W2010334049 creator A5012669178 @default.
- W2010334049 creator A5022593279 @default.
- W2010334049 creator A5022802907 @default.
- W2010334049 creator A5025489494 @default.
- W2010334049 creator A5032736520 @default.
- W2010334049 creator A5037859887 @default.
- W2010334049 creator A5041713354 @default.
- W2010334049 creator A5053909568 @default.
- W2010334049 creator A5054667253 @default.
- W2010334049 creator A5058885925 @default.
- W2010334049 creator A5062690734 @default.
- W2010334049 creator A5067557828 @default.
- W2010334049 creator A5069840499 @default.
- W2010334049 creator A5079565231 @default.
- W2010334049 creator A5084629463 @default.
- W2010334049 creator A5090513464 @default.
- W2010334049 creator A5052961630 @default.
- W2010334049 date "2013-04-21" @default.
- W2010334049 modified "2023-09-25" @default.
- W2010334049 title "QF-PCR as a substitute for karyotyping of cytotrophoblast for the analysis of chorionic villi: advantages and limitations from a cytogenetic retrospective audit of 44,727 first-trimester prenatal diagnoses" @default.
- W2010334049 cites W1924162700 @default.
- W2010334049 cites W1975386181 @default.
- W2010334049 cites W1984859328 @default.
- W2010334049 cites W1988773194 @default.
- W2010334049 cites W1996938815 @default.
- W2010334049 cites W2010846480 @default.
- W2010334049 cites W2013613602 @default.
- W2010334049 cites W2021905950 @default.
- W2010334049 cites W2025196417 @default.
- W2010334049 cites W2031800439 @default.
- W2010334049 cites W2038007215 @default.
- W2010334049 cites W2038214552 @default.
- W2010334049 cites W2040269748 @default.
- W2010334049 cites W2053837544 @default.
- W2010334049 cites W2069394435 @default.
- W2010334049 cites W2080616556 @default.
- W2010334049 cites W2088559557 @default.
- W2010334049 cites W2096058725 @default.
- W2010334049 cites W2100277762 @default.
- W2010334049 cites W2101613259 @default.
- W2010334049 cites W2101931336 @default.
- W2010334049 cites W2109823583 @default.
- W2010334049 cites W2131159740 @default.
- W2010334049 cites W2157656121 @default.
- W2010334049 cites W2160876667 @default.
- W2010334049 cites W4230901005 @default.
- W2010334049 cites W4243524184 @default.
- W2010334049 doi "https://doi.org/10.1002/pd.4099" @default.
- W2010334049 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24590588" @default.
- W2010334049 hasPublicationYear "2013" @default.
- W2010334049 type Work @default.
- W2010334049 sameAs 2010334049 @default.
- W2010334049 citedByCount "14" @default.
- W2010334049 countsByYear W20103340492013 @default.
- W2010334049 countsByYear W20103340492014 @default.
- W2010334049 countsByYear W20103340492015 @default.
- W2010334049 countsByYear W20103340492016 @default.
- W2010334049 countsByYear W20103340492017 @default.
- W2010334049 countsByYear W20103340492021 @default.
- W2010334049 countsByYear W20103340492022 @default.
- W2010334049 crossrefType "journal-article" @default.
- W2010334049 hasAuthorship W2010334049A5004851952 @default.
- W2010334049 hasAuthorship W2010334049A5010977968 @default.
- W2010334049 hasAuthorship W2010334049A5012669178 @default.
- W2010334049 hasAuthorship W2010334049A5022593279 @default.
- W2010334049 hasAuthorship W2010334049A5022802907 @default.
- W2010334049 hasAuthorship W2010334049A5025489494 @default.
- W2010334049 hasAuthorship W2010334049A5032736520 @default.
- W2010334049 hasAuthorship W2010334049A5037859887 @default.
- W2010334049 hasAuthorship W2010334049A5041713354 @default.
- W2010334049 hasAuthorship W2010334049A5052961630 @default.
- W2010334049 hasAuthorship W2010334049A5053909568 @default.
- W2010334049 hasAuthorship W2010334049A5054667253 @default.
- W2010334049 hasAuthorship W2010334049A5058885925 @default.
- W2010334049 hasAuthorship W2010334049A5062690734 @default.
- W2010334049 hasAuthorship W2010334049A5067557828 @default.
- W2010334049 hasAuthorship W2010334049A5069840499 @default.
- W2010334049 hasAuthorship W2010334049A5079565231 @default.
- W2010334049 hasAuthorship W2010334049A5084629463 @default.
- W2010334049 hasAuthorship W2010334049A5090513464 @default.
- W2010334049 hasConcept C104317684 @default.
- W2010334049 hasConcept C131872663 @default.
- W2010334049 hasConcept C142724271 @default.
- W2010334049 hasConcept C167135981 @default.
- W2010334049 hasConcept C172680121 @default.
- W2010334049 hasConcept C2776229224 @default.
- W2010334049 hasConcept C2776555163 @default.
- W2010334049 hasConcept C2776953305 @default.
- W2010334049 hasConcept C2777605040 @default.
- W2010334049 hasConcept C2778258057 @default.
- W2010334049 hasConcept C2779234561 @default.
- W2010334049 hasConcept C2780343435 @default.
- W2010334049 hasConcept C2781385326 @default.
- W2010334049 hasConcept C29456083 @default.