FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010356257> ?p ?o ?g. }

• W2010356257 endingPage "129" @default.
• W2010356257 startingPage "124" @default.
• W2010356257 abstract "Ion-pair reversed-phase high-performance liquid chromatography online hyphenated to electrospray ionization mass spectrometry (ICEMS) represents an efficient method for the characterization of nucleic acids amplified by polymerase chain reaction (PCR). Since sample preparation is limited to PCR, the optimization of its solution conditions is of utmost importance for efficient mass spectrometric detection. The compatibility of a number of different commercially available PCR components including DNA polymerases, deoxynucleotide triphosphates, bovine serum albumin, enhancer, and ionic buffers was evaluated. These experiments revealed that higher concentration of enhancer and detergents such as Tween-20 or Nonidet P-40 impairs the mass spectrometric detection of nucleic acids and should be avoided within the PCR mixture. The optimized analytical platform was applied to the characterization of PCR products covering parts of the first hypervariable region of the noncoding mitochondrial control region. Truncated amplicons were detected attributable to the use of low quality primers. Furthermore, due to the proofreading activity of the applied polymerase system, mismatches between the primer and the target sequence located at the last or the second last base at the 3'-end of primers were corrected and detected within the corresponding amplicons." @default.
• W2010356257 created "2016-06-24" @default.
• W2010356257 creator A5007975306 @default.
• W2010356257 creator A5018138430 @default.
• W2010356257 creator A5064305101 @default.
• W2010356257 creator A5079263249 @default.
• W2010356257 date "2006-02-01" @default.
• W2010356257 modified "2023-10-01" @default.
• W2010356257 title "Some guidelines for the analysis of genomic DNA by PCR-LC-ESI-MS" @default.
• W2010356257 cites W1599067438 @default.
• W2010356257 cites W1964082805 @default.
• W2010356257 cites W1994106895 @default.
• W2010356257 cites W1994324159 @default.
• W2010356257 cites W2003830057 @default.
• W2010356257 cites W2030698197 @default.
• W2010356257 cites W2035305815 @default.
• W2010356257 cites W2039142979 @default.
• W2010356257 cites W2042714411 @default.
• W2010356257 cites W2051679931 @default.
• W2010356257 cites W2086466506 @default.
• W2010356257 cites W2092512258 @default.
• W2010356257 cites W2092703454 @default.
• W2010356257 cites W2115589196 @default.
• W2010356257 cites W2134710898 @default.
• W2010356257 cites W2138270253 @default.
• W2010356257 cites W2146664247 @default.
• W2010356257 cites W2170141752 @default.
• W2010356257 cites W4239460767 @default.
• W2010356257 doi "https://doi.org/10.1016/j.jasms.2005.09.012" @default.
• W2010356257 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16406562" @default.
• W2010356257 hasPublicationYear "2006" @default.
• W2010356257 type Work @default.
• W2010356257 sameAs 2010356257 @default.
• W2010356257 citedByCount "17" @default.
• W2010356257 countsByYear W20103562572012 @default.
• W2010356257 countsByYear W20103562572013 @default.
• W2010356257 countsByYear W20103562572014 @default.
• W2010356257 countsByYear W20103562572016 @default.
• W2010356257 crossrefType "journal-article" @default.
• W2010356257 hasAuthorship W2010356257A5007975306 @default.
• W2010356257 hasAuthorship W2010356257A5018138430 @default.
• W2010356257 hasAuthorship W2010356257A5064305101 @default.
• W2010356257 hasAuthorship W2010356257A5079263249 @default.
• W2010356257 hasConcept C104317684 @default.
• W2010356257 hasConcept C153911025 @default.
• W2010356257 hasConcept C162356407 @default.
• W2010356257 hasConcept C17757408 @default.
• W2010356257 hasConcept C178790620 @default.
• W2010356257 hasConcept C185592680 @default.
• W2010356257 hasConcept C186008079 @default.
• W2010356257 hasConcept C24107716 @default.
• W2010356257 hasConcept C2777563447 @default.
• W2010356257 hasConcept C40684141 @default.
• W2010356257 hasConcept C43617362 @default.
• W2010356257 hasConcept C49105822 @default.
• W2010356257 hasConcept C52001828 @default.
• W2010356257 hasConcept C552990157 @default.
• W2010356257 hasConcept C55493867 @default.
• W2010356257 hasConcept C8185291 @default.
• W2010356257 hasConcept C82381507 @default.
• W2010356257 hasConcept C86803240 @default.
• W2010356257 hasConcept C86807702 @default.
• W2010356257 hasConcept C90583042 @default.
• W2010356257 hasConceptScore W2010356257C104317684 @default.
• W2010356257 hasConceptScore W2010356257C153911025 @default.
• W2010356257 hasConceptScore W2010356257C162356407 @default.
• W2010356257 hasConceptScore W2010356257C17757408 @default.
• W2010356257 hasConceptScore W2010356257C178790620 @default.
• W2010356257 hasConceptScore W2010356257C185592680 @default.
• W2010356257 hasConceptScore W2010356257C186008079 @default.
• W2010356257 hasConceptScore W2010356257C24107716 @default.
• W2010356257 hasConceptScore W2010356257C2777563447 @default.
• W2010356257 hasConceptScore W2010356257C40684141 @default.
• W2010356257 hasConceptScore W2010356257C43617362 @default.
• W2010356257 hasConceptScore W2010356257C49105822 @default.
• W2010356257 hasConceptScore W2010356257C52001828 @default.
• W2010356257 hasConceptScore W2010356257C552990157 @default.
• W2010356257 hasConceptScore W2010356257C55493867 @default.
• W2010356257 hasConceptScore W2010356257C8185291 @default.
• W2010356257 hasConceptScore W2010356257C82381507 @default.
• W2010356257 hasConceptScore W2010356257C86803240 @default.
• W2010356257 hasConceptScore W2010356257C86807702 @default.
• W2010356257 hasConceptScore W2010356257C90583042 @default.
• W2010356257 hasIssue "2" @default.
• W2010356257 hasLocation W20103562571 @default.
• W2010356257 hasLocation W20103562572 @default.
• W2010356257 hasOpenAccess W2010356257 @default.
• W2010356257 hasPrimaryLocation W20103562571 @default.
• W2010356257 hasRelatedWork W2001725983 @default.
• W2010356257 hasRelatedWork W2004027931 @default.
• W2010356257 hasRelatedWork W2037189757 @default.
• W2010356257 hasRelatedWork W2080534576 @default.
• W2010356257 hasRelatedWork W2093114369 @default.
• W2010356257 hasRelatedWork W2117564247 @default.
• W2010356257 hasRelatedWork W2143351325 @default.
• W2010356257 hasRelatedWork W2800731892 @default.
• W2010356257 hasRelatedWork W2808665756 @default.
• W2010356257 hasRelatedWork W423554223 @default.

• next