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- W2010365526 abstract "An intracellular 6G-fructofuranosidase (endo-type enzyme) extracted from Xanthophyllomyces dendrorhous 269 efficiently hydrolyzes fructosyl-β-(2→1)-linked sucrose to produce neo-kestose as a main transglycosylation product. The enzyme with a molecular weight of 33 kDa was purified by DEAE-52 cellulose chromatography. Thirty-fivefold purification and a 13.4% enzyme activity recovery were achieved. Optimum enzyme activity occurred at pH 6.4 and 45 °C and the enzyme was stable at pH 4–7 and at 45 °C. Using sucrose as a substrate, the Km and Vmax values were, respectively, 511 mmol/l and 233 μmol/(min mg) for transfer activity and 62 mmol/l and 164 μmol/(min mg) for hydrolytic activity. Under optimum conditions, a maximum concentration (73.9 g/l) of neo-fructooligosaccharides catalyzed by the endo-enzyme was obtained. These findings suggest that the purified endo-enzyme exhibits a high transfructosylation activity and it has potential for the industrial production of neo-FOSs." @default.
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- W2010365526 date "2011-01-01" @default.
- W2010365526 modified "2023-10-16" @default.
- W2010365526 title "Biochemical characterization of an intracellular 6G-fructofuranosidase from Xanthophyllomyces dendrorhous and its use in production of neo-fructooligosaccharides (neo-FOSs)" @default.
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- W2010365526 doi "https://doi.org/10.1016/j.biortech.2010.08.033" @default.
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