FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010366851> ?p ?o ?g. }

• W2010366851 endingPage "334" @default.
• W2010366851 startingPage "323" @default.
• W2010366851 abstract "Synaptic glycine levels are controlled by GLYTs (glycine transporters). GLYT1 is the main regulator of synaptic glycine concentrations and catalyses Na+-Cl--glycine co-transport with a 2:1:1 stoichiometry. In contrast, neuronal GLYT2 supplies glycine to the presynaptic terminal with a 3:1:1 stoichiometry. We subjected homology models of GLYT1 and GLYT2 to molecular dynamics simulations in the presence of Na+. Using molecular interaction potential maps and in silico mutagenesis, we identified a conserved region in the GLYT2 external vestibule likely to be involved in Na+ interactions. Replacement of Asp471 in this region reduced Na+ affinity and Na+ co-operativity of transport, an effect not produced in the homologous position (Asp295) in GLYT1. Unlike the GLYT1-Asp295 mutation, this Asp471 mutant increased sodium leakage and non-stoichiometric uncoupled ion movements through GLYT2, as determined by simultaneously measuring current and [3H]glycine accumulation. The homologous Asp471 and Asp295 positions exhibited distinct cation-sensitive external accessibility, and they were involved in Na+ and Li+-induced conformational changes. Although these two cations had opposite effects on GLYT1, they had comparable effects on accessibility in GLYT2, explaining the inhibitory and stimulatory responses to lithium exhibited by the two transporters. On the basis of these findings, we propose a role for Asp471 in controlling cation access to GLYT2 Na+ sites, ion coupling during transport and the subsequent conformational changes." @default.
• W2010366851 created "2016-06-24" @default.
• W2010366851 creator A5006456068 @default.
• W2010366851 creator A5008486598 @default.
• W2010366851 creator A5018154249 @default.
• W2010366851 creator A5023692978 @default.
• W2010366851 creator A5031725151 @default.
• W2010366851 creator A5036020802 @default.
• W2010366851 creator A5038356139 @default.
• W2010366851 creator A5044721653 @default.
• W2010366851 creator A5053606829 @default.
• W2010366851 creator A5071129392 @default.
• W2010366851 creator A5077704069 @default.
• W2010366851 creator A5089651692 @default.
• W2010366851 date "2012-02-13" @default.
• W2010366851 modified "2023-10-18" @default.
• W2010366851 title "An aspartate residue in the external vestibule of GLYT2 (glycine transporter 2) controls cation access and transport coupling" @default.
• W2010366851 cites W1578249395 @default.
• W2010366851 cites W1592442520 @default.
• W2010366851 cites W1799612632 @default.
• W2010366851 cites W1967945001 @default.
• W2010366851 cites W1969174524 @default.
• W2010366851 cites W1970870670 @default.
• W2010366851 cites W1971264177 @default.
• W2010366851 cites W1977084918 @default.
• W2010366851 cites W1983636667 @default.
• W2010366851 cites W1985903963 @default.
• W2010366851 cites W1987110948 @default.
• W2010366851 cites W1988664275 @default.
• W2010366851 cites W1989340741 @default.
• W2010366851 cites W1992415594 @default.
• W2010366851 cites W1994522080 @default.
• W2010366851 cites W1995875445 @default.
• W2010366851 cites W1996517727 @default.
• W2010366851 cites W1999895476 @default.
• W2010366851 cites W2005533602 @default.
• W2010366851 cites W2019974245 @default.
• W2010366851 cites W2029582401 @default.
• W2010366851 cites W2029667189 @default.
• W2010366851 cites W2032018593 @default.
• W2010366851 cites W2041499969 @default.
• W2010366851 cites W2043894152 @default.
• W2010366851 cites W2049591302 @default.
• W2010366851 cites W2050453104 @default.
• W2010366851 cites W2052242727 @default.
• W2010366851 cites W2056960549 @default.
• W2010366851 cites W2056982651 @default.
• W2010366851 cites W2065283382 @default.
• W2010366851 cites W2072465203 @default.
• W2010366851 cites W2077531668 @default.
• W2010366851 cites W2077682188 @default.
• W2010366851 cites W2079382471 @default.
• W2010366851 cites W2085728548 @default.
• W2010366851 cites W2091260123 @default.
• W2010366851 cites W2094107471 @default.
• W2010366851 cites W2104622728 @default.
• W2010366851 cites W2134596683 @default.
• W2010366851 cites W2134765462 @default.
• W2010366851 cites W2150926065 @default.
• W2010366851 cites W2150981663 @default.
• W2010366851 cites W2151831732 @default.
• W2010366851 cites W2153562609 @default.
• W2010366851 cites W2158714788 @default.
• W2010366851 cites W2165917097 @default.
• W2010366851 doi "https://doi.org/10.1042/bj20110247" @default.
• W2010366851 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22132725" @default.
• W2010366851 hasPublicationYear "2012" @default.
• W2010366851 type Work @default.
• W2010366851 sameAs 2010366851 @default.
• W2010366851 citedByCount "17" @default.
• W2010366851 countsByYear W20103668512012 @default.
• W2010366851 countsByYear W20103668512013 @default.
• W2010366851 countsByYear W20103668512014 @default.
• W2010366851 countsByYear W20103668512015 @default.
• W2010366851 countsByYear W20103668512016 @default.
• W2010366851 countsByYear W20103668512017 @default.
• W2010366851 countsByYear W20103668512018 @default.
• W2010366851 countsByYear W20103668512019 @default.
• W2010366851 countsByYear W20103668512021 @default.
• W2010366851 countsByYear W20103668512022 @default.
• W2010366851 countsByYear W20103668512023 @default.
• W2010366851 crossrefType "journal-article" @default.
• W2010366851 hasAuthorship W2010366851A5006456068 @default.
• W2010366851 hasAuthorship W2010366851A5008486598 @default.
• W2010366851 hasAuthorship W2010366851A5018154249 @default.
• W2010366851 hasAuthorship W2010366851A5023692978 @default.
• W2010366851 hasAuthorship W2010366851A5031725151 @default.
• W2010366851 hasAuthorship W2010366851A5036020802 @default.
• W2010366851 hasAuthorship W2010366851A5038356139 @default.
• W2010366851 hasAuthorship W2010366851A5044721653 @default.
• W2010366851 hasAuthorship W2010366851A5053606829 @default.
• W2010366851 hasAuthorship W2010366851A5071129392 @default.
• W2010366851 hasAuthorship W2010366851A5077704069 @default.
• W2010366851 hasAuthorship W2010366851A5089651692 @default.
• W2010366851 hasBestOaLocation W20103668512 @default.
• W2010366851 hasConcept C104317684 @default.
• W2010366851 hasConcept C12554922 @default.
• W2010366851 hasConcept C149011108 @default.

• next