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- W2010379718 abstract "Early investigations (Roberts, Lowe, Guth and Jelinek, 1958) of the ninhydrin positive substances of the whole brain of rats indicated that these substances were unchanged by various drug treatments or physiological stresses. It was mentioned at that time that studies of the whole brain could be misleading. Later work with whole brains of rats and mice (Häkkinen and Kulonen, 1961; Soep and Janssen, 1961; DeRopp and Snedeker, 1961; Kamrin and Kamrin, 1961) revealed that the administration of different drugs does alter the concentration of several of the free amino acids. Although no meaningful pattern of changes was evident, these reports raised the possibility that a meaningful pattern of changes could be found through a more detailed study with more refined techniques. In recent years the development of dual column (Spackman, Stein and Moore, 1958) and single column (Piez and Morris, 1960) amino acid analysers has facilitated the relatively rapid separation and determination of ninhydrin positive substances in biological extracts. The studies of Tallan, Moore and Stein (1954) had already established the large number of ninhydrin positive substances present in the brain of a cat as revealed by column chromatography, including unknown peaks. This paper describes in detail the procedures used in establishing the concentrations of ninhydrin positive substances in four areas of the rat brain. Other body organs and tissues were analysed for comparison to establish the presence of unknown substances peculiar to the brain." @default.
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- W2010379718 date "1965-03-01" @default.
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- W2010379718 title "NINHYDRIN POSITIVE SUBSTANCES PRESENT IN DIFFERENT AREAS OF NORMAL RAT BRAIN" @default.
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- W2010379718 doi "https://doi.org/10.1111/j.1471-4159.1965.tb06751.x" @default.
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