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- W2010388669 abstract "A rapid method for the purification of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase from the livers of cholestyramine-fed rats is reported. The procedure involves a sequence of separations on affinity chromatography columns consisting of Blue Dextran-Sepharose, agarose-CoA, and agarose-HMG-CoA. The advantage of this method is its flexibility in scavenging enzyme that might be lost during purification, resulting in a yield of homogeneous reductase (specific activity approximately 10,000 nmol/min/mg protein) as high as 50%, which is at least twice that previously reported." @default.
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- W2010388669 title "Rapid, high-yield purification of rat liver 3-hydroxy-3-methyl-glutaryl-coenzyme a reductase" @default.
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- W2010388669 doi "https://doi.org/10.1016/0003-2697(80)90047-0" @default.
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