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- W2010393386 abstract "The amount of phosphate in soil microbial biomass was estimated by adding CHCl3 to soil to lyse microbial cells and measuring the proportion of microbial-P released to 0.5 M NaHCO3 extracts (pH 8.5). Calculations of total microbial-P were based on the difference between P removed by NaHCO3 extraction ofChCl3 treated and untreated samples. This method was improved by (1) removing resin-extractable P from the soil before lysing microbial cells with CHCl3, (2) measuring total P rather than inorganic-P in NaHCO3 extracts, and (3) prolonging NaHCO3 extraction from 30 min to 16 h. The efficiency of extraction of soil microbial-P was determined by measuring the P recovered from two species each of bacteria and fungi grown first in solutions having different P concentrations, then added to soil. The average proportion of microbial-P recovered from a neutral calcareous soil was 37% (KP = 0.37). The Kp factor was confirmed by adding 33P-labelled bacteria to a similar soil and measuring the added 33P recovered (38%) by the CHCl3-NaHCO3 method." @default.
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- W2010393386 date "1982-01-01" @default.
- W2010393386 modified "2023-09-30" @default.
- W2010393386 title "Method to measure microbial phosphate in soils" @default.
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- W2010393386 doi "https://doi.org/10.1016/0038-0717(82)90009-8" @default.
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