FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010397795> ?p ?o ?g. }

• W2010397795 endingPage "689" @default.
• W2010397795 startingPage "683" @default.
• W2010397795 abstract "The possible reversibility of pH induced activation of the glucocorticoid-receptor complex was studied. Generally, this was accomplished by activating rat liver cytosol at pH 8.5 (15 degrees C, 30 min), and then returning it to pH 6.5 for a second incubation (15 degrees C, 30 min). Activation was quantitated by measuring the binding of [3H]triamcinolone acetonide [( 3H]TA)-receptor complexes to DNA-cellulose. When cytosol was incubated at pH 6.5, only 4.1% of the [3H]TA-receptor complexes bound to DNA-cellulose. However, 39.2% of the complexes bound when the cytosol was pH activated. When pH activation was followed by a second incubation at pH 6.5, 47.0% of the steroid-receptor complexes bound. Thus, according to the DNA-cellulose binding assay, pH induced activation was irreversible. In order to visualize both activated and unactivated [3H]TA-receptor complexes during this process, diethylaminoethyl (DEAE)-cellulose chromatography was performed. When cytosol was incubated at pH 6.5, only 19.6% of the [3H]TA-receptor complexes were eluted in the activated form from DEAE-cellulose. However, 67.5% of the complexes were eluted in the activated form when cytosol was pH activated. When pH activation was followed by a second incubation at pH 6.5, 74.9% of the steroid-receptor complexes were eluted in the activated form. Thus, DEAE-cellulose chromatography also showed that pH induced activation was irreversible. This is the first known report that the combination of DNA-cellulose binding and DEAE-cellulose chromatography have been used to study pH induced activation of the glucocorticoid-receptor complex. By these criteria, we conclude that in vitro pH induced activation is irreversible." @default.
• W2010397795 created "2016-06-24" @default.
• W2010397795 creator A5021968564 @default.
• W2010397795 creator A5052911594 @default.
• W2010397795 creator A5062814155 @default.
• W2010397795 date "1984-03-01" @default.
• W2010397795 modified "2023-09-23" @default.
• W2010397795 title "Evidence that pH induced activation of the rat hepatic glucocorticoid-receptor complex is irreversible" @default.
• W2010397795 cites W1481489488 @default.
• W2010397795 cites W1533986732 @default.
• W2010397795 cites W1548712478 @default.
• W2010397795 cites W1568015334 @default.
• W2010397795 cites W1568701525 @default.
• W2010397795 cites W1574229173 @default.
• W2010397795 cites W1593695748 @default.
• W2010397795 cites W1601029230 @default.
• W2010397795 cites W178378932 @default.
• W2010397795 cites W1881700477 @default.
• W2010397795 cites W1969197892 @default.
• W2010397795 cites W1979286967 @default.
• W2010397795 cites W1985313279 @default.
• W2010397795 cites W1985895340 @default.
• W2010397795 cites W1986106014 @default.
• W2010397795 cites W1995368714 @default.
• W2010397795 cites W1996453077 @default.
• W2010397795 cites W2014139892 @default.
• W2010397795 cites W2018632980 @default.
• W2010397795 cites W2022999461 @default.
• W2010397795 cites W2024098392 @default.
• W2010397795 cites W2039816246 @default.
• W2010397795 cites W2042851875 @default.
• W2010397795 cites W2046201653 @default.
• W2010397795 cites W2050586961 @default.
• W2010397795 cites W2073521402 @default.
• W2010397795 cites W2085128396 @default.
• W2010397795 cites W2089250490 @default.
• W2010397795 cites W2109746022 @default.
• W2010397795 cites W2208707498 @default.
• W2010397795 cites W2342073136 @default.
• W2010397795 doi "https://doi.org/10.1016/0022-4731(84)90071-2" @default.
• W2010397795 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6708546" @default.
• W2010397795 hasPublicationYear "1984" @default.
• W2010397795 type Work @default.
• W2010397795 sameAs 2010397795 @default.
• W2010397795 citedByCount "12" @default.
• W2010397795 crossrefType "journal-article" @default.
• W2010397795 hasAuthorship W2010397795A5021968564 @default.
• W2010397795 hasAuthorship W2010397795A5052911594 @default.
• W2010397795 hasAuthorship W2010397795A5062814155 @default.
• W2010397795 hasConcept C116569031 @default.
• W2010397795 hasConcept C170493617 @default.
• W2010397795 hasConcept C181199279 @default.
• W2010397795 hasConcept C185592680 @default.
• W2010397795 hasConcept C25642318 @default.
• W2010397795 hasConcept C2779251873 @default.
• W2010397795 hasConcept C43617362 @default.
• W2010397795 hasConcept C55493867 @default.
• W2010397795 hasConcept C59493245 @default.
• W2010397795 hasConcept C73826308 @default.
• W2010397795 hasConcept C98539663 @default.
• W2010397795 hasConceptScore W2010397795C116569031 @default.
• W2010397795 hasConceptScore W2010397795C170493617 @default.
• W2010397795 hasConceptScore W2010397795C181199279 @default.
• W2010397795 hasConceptScore W2010397795C185592680 @default.
• W2010397795 hasConceptScore W2010397795C25642318 @default.
• W2010397795 hasConceptScore W2010397795C2779251873 @default.
• W2010397795 hasConceptScore W2010397795C43617362 @default.
• W2010397795 hasConceptScore W2010397795C55493867 @default.
• W2010397795 hasConceptScore W2010397795C59493245 @default.
• W2010397795 hasConceptScore W2010397795C73826308 @default.
• W2010397795 hasConceptScore W2010397795C98539663 @default.
• W2010397795 hasIssue "3" @default.
• W2010397795 hasLocation W20103977951 @default.
• W2010397795 hasLocation W20103977952 @default.
• W2010397795 hasOpenAccess W2010397795 @default.
• W2010397795 hasPrimaryLocation W20103977951 @default.
• W2010397795 hasRelatedWork W1512627224 @default.
• W2010397795 hasRelatedWork W1972701187 @default.
• W2010397795 hasRelatedWork W2008464425 @default.
• W2010397795 hasRelatedWork W2072641425 @default.
• W2010397795 hasRelatedWork W2080189603 @default.
• W2010397795 hasRelatedWork W2404942261 @default.
• W2010397795 hasRelatedWork W2417715046 @default.
• W2010397795 hasRelatedWork W2433748563 @default.
• W2010397795 hasRelatedWork W2468069532 @default.
• W2010397795 hasRelatedWork W2068888253 @default.
• W2010397795 hasVolume "20" @default.
• W2010397795 isParatext "false" @default.
• W2010397795 isRetracted "false" @default.
• W2010397795 magId "2010397795" @default.
• W2010397795 workType "article" @default.