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• W2010400539 endingPage "9001" @default.
• W2010400539 startingPage "8987" @default.
• W2010400539 abstract "Cytokinesis partitions the cytoplasm of a parent cell into two daughter cells and is essential for the completion of cell division. The final step of cytokinesis in animal cells is abscission, which is a process leading to the physical separation of two daughter cells. Abscission requires membrane traffic and microtubule disassembly at a specific midbody region called the secondary ingression. Here, we report that WD repeat-containing protein 5 (WDR5), a core subunit of COMPASS/MLL family histone H3 lysine 4 methyltransferase (H3K4MT) complexes, resides at the midbody and associates with a subset of midbody regulatory proteins, including PRC1 and CYK4/MKLP1. Knockdown of WDR5 impairs abscission and increases the incidence of multinucleated cells. Further investigation revealed that the abscission delay is primarily due to slower formation of secondary ingressions in WDR5 knockdown cells. Consistent with these defects, midbody microtubules in WDR5 knockdown cells also display enhanced resistance to depolymerization by nocodazole. Recruitment of WDR5 to the midbody dark zone appears to require integrity of the WDR5 central arginine-binding cavity, as mutations that disrupt histone H3 and MLL1 binding to this pocket also abolish the midbody localization of WDR5. Taken together, these data suggest that WDR5 is specifically targeted to the midbody in the absence of chromatin and that it promotes abscission, perhaps by facilitating midbody microtubule disassembly. Cytokinesis partitions the cytoplasm of a parent cell into two daughter cells and is essential for the completion of cell division. The final step of cytokinesis in animal cells is abscission, which is a process leading to the physical separation of two daughter cells. Abscission requires membrane traffic and microtubule disassembly at a specific midbody region called the secondary ingression. Here, we report that WD repeat-containing protein 5 (WDR5), a core subunit of COMPASS/MLL family histone H3 lysine 4 methyltransferase (H3K4MT) complexes, resides at the midbody and associates with a subset of midbody regulatory proteins, including PRC1 and CYK4/MKLP1. Knockdown of WDR5 impairs abscission and increases the incidence of multinucleated cells. Further investigation revealed that the abscission delay is primarily due to slower formation of secondary ingressions in WDR5 knockdown cells. Consistent with these defects, midbody microtubules in WDR5 knockdown cells also display enhanced resistance to depolymerization by nocodazole. Recruitment of WDR5 to the midbody dark zone appears to require integrity of the WDR5 central arginine-binding cavity, as mutations that disrupt histone H3 and MLL1 binding to this pocket also abolish the midbody localization of WDR5. Taken together, these data suggest that WDR5 is specifically targeted to the midbody in the absence of chromatin and that it promotes abscission, perhaps by facilitating midbody microtubule disassembly. WD repeat-containing protein 5 (WDR5) localizes to the midbody and regulates abscission.Journal of Biological ChemistryVol. 290Issue 37PreviewVOLUME 290 (2015) PAGES 8987–9001 Full-Text PDF Open Access" @default.
• W2010400539 created "2016-06-24" @default.
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• W2010400539 creator A5054869714 @default.
• W2010400539 date "2015-04-01" @default.
• W2010400539 modified "2023-10-15" @default.
• W2010400539 title "WD Repeat-containing Protein 5 (WDR5) Localizes to the Midbody and Regulates Abscission" @default.
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• W2010400539 doi "https://doi.org/10.1074/jbc.m114.623611" @default.
• W2010400539 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4566220" @default.
• W2010400539 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26362543" @default.
• W2010400539 hasPublicationYear "2015" @default.

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