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- W2010412967 abstract "This paper describes a simple and reliable method for the solubilization of rat liver 3-hydroxy-3-methylglutaryl coenzyme A reductase by extracting lyophilized microsomes with buffer. This procedure is suitable for the preparation of large quantities of soluble enzyme and does not require the use of organic solvents, detergents or other stringent conditions which might alter the properties of the enzyme. A test of cold lability in 3-hydroxy-3-methylglutaryl coenzyme A reductase solutions is described. No substantial cold lability was observed. These results indicate that the sensitivity to cold reported by other investigators probably is not an inherent property of the enzyme." @default.
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- W2010412967 date "1974-01-01" @default.
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- W2010412967 title "Solubilization of 3-hydroxy-3-methylglutaryl coenzyme A reductase from lyophilized rat liver microsomes: Lack of evidence for cold lability in this soluble enzyme preparation" @default.
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- W2010412967 doi "https://doi.org/10.1016/s0006-291x(74)80311-6" @default.
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