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• W2010432717 abstract "Abstract 1. 1. In a correlated biochemical and morphological study, changes in the activity and subcellular distribution of acid hydrolases were investiagted and related to previous observations of changes in phospholipids and formation of vacuoles in HeLa cells exposed to immunoglobulin G (IgG) and complement. 2. 2. In cells exposed to anti-HeLa cell immunoglobulin G (anti-HeLa IgG) and complement-sufficient guinea-pig serum the activity of arylsulphatase (aryl sulphate sulphohydrolase, EC3.1.6.1.) and acid phenyl phosphomonoesterase (acid orthophosphoric monoester phosphorylase, EC3.1.3.2.) showed a transient increase within half an hour. In cells exposed to anti-HeLa IgG or control IgG and heat-inactivated complement hydrolase activities increased within 2.5 h. 3. 3. The distribution of arylsulphate and acid phenyl phosphomonoesterase activities among the supernatant fluid and the corresponding pellet after centrifugation at 18800 × g form 21 min, showed, in cells exposed to anti-HeLa IgG and complement, a continuous increase in the activity of unsedimentable hydrolases, but a decrease in cells exposed to IgG and heat-inactivated serum. 4. 4. In cells exposed to anti-HeLa IgG and complement, thermal activation studies of the latency in release of vacuole-linked acid phenyl phosphomonoesterase showed an increase in enzyme release corresponding to the increase of unsedimentable acid phenyl phosphomonoesterase activity of these cells. 5. 5. The observed transient increase in activity of two acid hydrolases accepted as typical marker enzymes of lysosomes precedes the changes in lipids and appearance of vacuoles described previously in HeLa cells exposed to IgG and complement. A relatinship with respect to time was, however, demonstrated between the observed increase in release of unsedimentable acid hydrolases and the accumulation of lysophosphatidyl choline in these cells." @default.
• W2010432717 created "2016-06-24" @default.
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• W2010432717 date "1971-04-01" @default.
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• W2010432717 title "Correlated changes in acid hydrolase activity and membrane lipids of HeLa cells: Changes in activity and subcellular distribution of arylsulphatase and acid phenyl phosphomonoesterase" @default.
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• W2010432717 doi "https://doi.org/10.1016/0304-4165(71)90028-6" @default.
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