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- W2010450174 abstract "A facile and sensitive label-free approach for detection of thrombin based on CdTe quantum dots (QDs) and thrombin binding aptamer (TBA) is presented. The crude QDs can be activated with fluorescence enhancement by adding extra Cd(2+) to the solution in basic medium. As a result, the positively charged Cd(2+)-activating CdTe QDs could interact with the negatively charged TBA, leading to fluorescence quenching. When thrombin was added, TBA was induced to form a G-quadruplex structure and combined specifically with its target, releasing the QDs with a recovery of the fluorescence intensity. The sensing approach is based on the strongly specific interactions between TBA and thrombin over the electrostatic interactions between TBA and positively charged QDs. Based on the fluorescence enhancement of QDs, selective detection of thrombin was successfully achieved. A linear response for thrombin was observed in the range from 1.4 nM to 21 nM with a detection limit of 0.70 nM." @default.
- W2010450174 created "2016-06-24" @default.
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- W2010450174 date "2013-03-01" @default.
- W2010450174 modified "2023-09-26" @default.
- W2010450174 title "Label-free sensing of thrombin based on quantum dots and thrombin binding aptamer" @default.
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- W2010450174 doi "https://doi.org/10.1016/j.talanta.2013.01.003" @default.
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