FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010451824> ?p ?o ?g. }

• W2010451824 endingPage "444" @default.
• W2010451824 startingPage "435" @default.
• W2010451824 abstract "Quantitative reverse transcriptase polymerase chain reaction (RT–PCR) analysis has previously shown that the P2Y 14 receptor is expressed in peripheral immune cells including lymphocytes. Although in transfected cells the P2Y 14 receptor couples to pertussis toxin‐sensitive G i/o protein, the functional coupling of endogenously expressed P2Y 14 receptors to the inhibition of adenylyl cyclase activity has not been reported. Therefore, the primary aim of this study was to determine whether the P2Y 14 receptor is functionally expressed in murine spleen‐derived T‐ and B‐lymphocyte‐enriched populations. RT–PCR analysis detected the expression of P2Y 14 receptor mRNA in whole spleen and isolated T‐ and B‐lymphocytes. In T cells, UDP‐glucose (EC 50 =335 n M ) induced a small but significant inhibition ( circa 20%) of forskolin‐stimulated cAMP accumulation, suggesting functional coupling of endogenously expressed P2Y 14 receptors to the inhibition of adenylyl cyclase activity. In contrast, the other putative P2Y 14 receptor agonists UDP‐galactose, UDP‐glucuronic acid and UDP‐ N ‐acetylglucosamine had no significant effect alone but behaved as partial agonists by blocking UDP‐glucose responses. In B cells, UDP‐glucose (100 μ M ) had no significant effect on forskolin‐stimulated cAMP accumulation. Treatment of T cells with pertussis toxin (G i/o blocker) abolished the inhibitory effects of UDP‐glucose on forskolin‐stimulated cAMP accumulation. T‐cell proliferation in response to anti‐CD3 monoclonal antibody (1 μ g ml −1 ) was significantly inhibited by UDP‐glucose (59% inhibition; p[IC 50 ]=5.9±0.3), UDP‐ N ‐acetylglucosamine (37%; 6.1±0.3), UDP‐galactose (56%; 8.2±0.2) and UDP‐glucuronic acid (49%; 6.3±0.2). Interleukin‐2‐ (5 ng ml −1 ) induced T‐cell proliferation was also significantly inhibited by all four agonists. In summary, we have shown that the P2Y 14 receptor appears to be functionally expressed in murine spleen‐derived T‐lymphocytes. These observations suggest that UDP‐glucose and related sugar nucleotides presumably via the P2Y 14 receptor may play an important role in modulating immune function. British Journal of Pharmacology (2005) 146 , 435–444. doi: 10.1038/sj.bjp.0706322" @default.
• W2010451824 created "2016-06-24" @default.
• W2010451824 creator A5034862572 @default.
• W2010451824 creator A5062048705 @default.
• W2010451824 date "2005-10-01" @default.
• W2010451824 modified "2023-10-16" @default.
• W2010451824 title "Functional expression of the P2Y₁₄ receptor in murine T-lymphocytes" @default.
• W2010451824 cites W161060649 @default.
• W2010451824 cites W1611136214 @default.
• W2010451824 cites W1895559133 @default.
• W2010451824 cites W1969866442 @default.
• W2010451824 cites W1979942820 @default.
• W2010451824 cites W1995160887 @default.
• W2010451824 cites W2001040603 @default.
• W2010451824 cites W2012663360 @default.
• W2010451824 cites W2015022932 @default.
• W2010451824 cites W2015109577 @default.
• W2010451824 cites W2019352531 @default.
• W2010451824 cites W2019527305 @default.
• W2010451824 cites W2028509040 @default.
• W2010451824 cites W2029257586 @default.
• W2010451824 cites W2049819186 @default.
• W2010451824 cites W2050417355 @default.
• W2010451824 cites W2068767330 @default.
• W2010451824 cites W2081050475 @default.
• W2010451824 cites W2086834997 @default.
• W2010451824 cites W2102223391 @default.
• W2010451824 cites W2114710340 @default.
• W2010451824 cites W2146718841 @default.
• W2010451824 cites W2153875468 @default.
• W2010451824 cites W2409038462 @default.
• W2010451824 cites W4292410652 @default.
• W2010451824 doi "https://doi.org/10.1038/sj.bjp.0706322" @default.
• W2010451824 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1576276" @default.
• W2010451824 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15997228" @default.
• W2010451824 hasPublicationYear "2005" @default.
• W2010451824 type Work @default.
• W2010451824 sameAs 2010451824 @default.
• W2010451824 citedByCount "58" @default.
• W2010451824 countsByYear W20104518242012 @default.
• W2010451824 countsByYear W20104518242013 @default.
• W2010451824 countsByYear W20104518242014 @default.
• W2010451824 countsByYear W20104518242015 @default.
• W2010451824 countsByYear W20104518242016 @default.
• W2010451824 countsByYear W20104518242017 @default.
• W2010451824 countsByYear W20104518242018 @default.
• W2010451824 countsByYear W20104518242019 @default.
• W2010451824 countsByYear W20104518242020 @default.
• W2010451824 countsByYear W20104518242021 @default.
• W2010451824 countsByYear W20104518242022 @default.
• W2010451824 countsByYear W20104518242023 @default.
• W2010451824 crossrefType "journal-article" @default.
• W2010451824 hasAuthorship W2010451824A5034862572 @default.
• W2010451824 hasAuthorship W2010451824A5062048705 @default.
• W2010451824 hasBestOaLocation W20104518242 @default.
• W2010451824 hasConcept C126322002 @default.
• W2010451824 hasConcept C134018914 @default.
• W2010451824 hasConcept C153911025 @default.
• W2010451824 hasConcept C170493617 @default.
• W2010451824 hasConcept C2779178603 @default.
• W2010451824 hasConcept C2779276759 @default.
• W2010451824 hasConcept C49773422 @default.
• W2010451824 hasConcept C55493867 @default.
• W2010451824 hasConcept C71924100 @default.
• W2010451824 hasConcept C80631254 @default.
• W2010451824 hasConcept C86803240 @default.
• W2010451824 hasConceptScore W2010451824C126322002 @default.
• W2010451824 hasConceptScore W2010451824C134018914 @default.
• W2010451824 hasConceptScore W2010451824C153911025 @default.
• W2010451824 hasConceptScore W2010451824C170493617 @default.
• W2010451824 hasConceptScore W2010451824C2779178603 @default.
• W2010451824 hasConceptScore W2010451824C2779276759 @default.
• W2010451824 hasConceptScore W2010451824C49773422 @default.
• W2010451824 hasConceptScore W2010451824C55493867 @default.
• W2010451824 hasConceptScore W2010451824C71924100 @default.
• W2010451824 hasConceptScore W2010451824C80631254 @default.
• W2010451824 hasConceptScore W2010451824C86803240 @default.
• W2010451824 hasIssue "3" @default.
• W2010451824 hasLocation W20104518241 @default.
• W2010451824 hasLocation W20104518242 @default.
• W2010451824 hasLocation W20104518243 @default.
• W2010451824 hasLocation W20104518244 @default.
• W2010451824 hasOpenAccess W2010451824 @default.
• W2010451824 hasPrimaryLocation W20104518241 @default.
• W2010451824 hasRelatedWork W1977982331 @default.
• W2010451824 hasRelatedWork W1980806930 @default.
• W2010451824 hasRelatedWork W1984957160 @default.
• W2010451824 hasRelatedWork W2019451990 @default.
• W2010451824 hasRelatedWork W2028910572 @default.
• W2010451824 hasRelatedWork W2043628569 @default.
• W2010451824 hasRelatedWork W2055820236 @default.
• W2010451824 hasRelatedWork W2150623384 @default.
• W2010451824 hasRelatedWork W2405405598 @default.
• W2010451824 hasRelatedWork W2418200535 @default.
• W2010451824 hasVolume "146" @default.
• W2010451824 isParatext "false" @default.
• W2010451824 isRetracted "false" @default.
• W2010451824 magId "2010451824" @default.

• next