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• W2010455679 abstract "Topographies of a bioactive glass (45S5 type Bioglass®) during 0–4 h of immersion in a supersaturated calcifying solution (SCS) and the SCS containing recombinant porcine amelogenin rP172 (SCSrP172) were observed by atomic force microscopy. Other techniques including X-ray diffraction, scanning electron microscopy coupled with energy dispersive X-ray spectroscopy, and transmission electron microscopy were used for some complementary microstructural investigations. The smooth Bioglass surface changed to be very rough after 0.5 h of SCS immersion because of glass network dissolution. Spherical silica-gel particles with diameters of 150–300 nm consisting of substructures of 20–60 nm across had formed on the sample surfaces after 1 h of SCS immersion. The chemisorption of amorphous calcium phosphate and crystallization of nanophase apatite were seen to occur epitaxially on the silica-gel structures during 1–4 h of SCS immersion. During the first 0.5 h of SCSrP172 immersion, more than 95% of rP172 protein in solution was adsorbed onto the sample surfaces and generated spherical assemblies of 10–60 nm diameters. During 0.5–4 h of SCSrP172 immersion, the protein assemblies of rP172 remarkably induced the formation of orientated silica-gel plates (approximately 100-nm wide and 50-nm thick) and subsequently of long and thin apatite needle crystals. The recombinant amelogenin rP172-modulated apatite crystals resembled those formed in the early stage of tooth enamel biomineralization, suggesting the functional roles of amelogenins during the oriented growth of enamel crystallites and a great potential for amelogenins in applications designed to fabricate enamel-like calcium phosphate biomaterials. © 2000 John Wiley & Sons, Inc. J Biomed Mater Res, 52, 762–773, 2000." @default.
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• W2010455679 date "2000-01-01" @default.
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• W2010455679 title "Effects of amelogenin on the transforming surface microstructures of Bioglass� in a calcifying solution" @default.
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• W2010455679 doi "https://doi.org/10.1002/1097-4636(20001215)52:4<762::aid-jbm22>3.0.co;2-w" @default.
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