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• W2010457940 abstract "No AccessJournal of UrologyInvestigative Urology1 Oct 1999EXPRESSION OF BIKUNIN mRNA IN RENAL EPITHELIAL CELLS AFTER OXALATE EXPOSURE SHIZUKA IIDA, AMMON B. PECK, KAREN J. BYER, and SAEED R. KHAN SHIZUKA IIDASHIZUKA IIDA More articles by this author , AMMON B. PECKAMMON B. PECK More articles by this author , KAREN J. BYERKAREN J. BYER More articles by this author , and SAEED R. KHANSAEED R. KHAN More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)68344-9AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: To determine the changes in expression of bikunin in renal epithelial cells on exposure to oxalate and calcium oxalate crystals. Materials and Methods: This study used reverse transcription polymerase chain reaction (RT-PCR) to examine bikunin mRNA expression levels in MDCK cells exposed to oxalate or calcium oxalate crystals. Poly(A)+ RNA was isolated directly from renal epithelial cells, then converted to cDNA with random primers and reverse transcriptase. To quantify the expression level of bikunin mRNA, we developed a competitive DNA template for competitive PCR analysis. The PCR products were resolved by electrophoresis on 1.3% agarose gel and visualized with ethidium bromide. In this system, we could quantify the exact number of bikunin mRNA transcripts. Bikunin mRNA from rat liver was expressed as a positive control. Results: Bikunin mRNAs and competitive templates from renal epithelial cells were expressed in all samples as 434 bp and 312 bp bands, respectively. Bikunin expression was significantly increased in oxalate exposed cells. Cells exposed to calcium oxalate monohydrate crystals or latex beads showed no significant change in expression of bikunin. Western blotting analysis also showed increased expression of bikunin and inter-α-inhibitor-related proteins in the culture medium of oxalate exposed cells. Conclusions: These findings suggest that renal epithelial cells express bikunin gene and have the capability to produce bikunin when stimulated by certain agents such as oxalate. This increased expression and production of bikunin may represent a protective response of renal epithelial cells to nephrotoxic challenges of oxalate. References 1 : Isolation and purification of a new glycoprotein from human urine inhibiting calcium oxalate crystallization. Urol. Res.1993; 21: 61. Google Scholar 2 : An unidentified macromolecular inhibitory constituent of calcium oxalate crystal growth in human urine. Urol. Res.1990; 18: 373. Google Scholar 3 : Characterization of uronic-acid-rich inhibitor of calcium oxalate crystalization isolated from rat urine. Urol. Res.1995; 23: 95. 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Google Scholar From the Department of Pathology and Laboratory Medicine, University of Florida College of Medicine, Gainesville, Florida© 1999 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byMORIYAMA M, GLENTON P and KHAN S (2018) EXPRESSION OF INTER-α INHIBITOR RELATED PROTEINS IN KIDNEYS AND URINE OF HYPEROXALURIC RATSJournal of Urology, VOL. 165, NO. 5, (1687-1692), Online publication date: 1-May-2001.GOKHALE J, GLENTON P and KHAN S (2018) CHARACTERIZATION OF TAMM-HORSFALL PROTEIN IN A RAT NEPHROLITHIASIS MODELJournal of Urology, VOL. 166, NO. 4, (1492-1497), Online publication date: 1-Oct-2001.TSUJIHATA M, MIYAKE O, YOSHIMURA K, KAKIMOTO K, TAKAHARA S and OKUYAMA A (2018) FIBRONECTIN AS A POTENT INHIBITOR OF CALCIUM OXALATE UROLITHIASISJournal of Urology, VOL. 164, NO. 5, (1718-1723), Online publication date: 1-Nov-2000. Volume 162Issue 4October 1999Page: 1480-1486 Advertisement Copyright & Permissions© 1999 by American Urological Association, Inc.Keywordsbikunincalcium oxalatekidney stonesinter-α-inhibitorMetricsAuthor Information SHIZUKA IIDA More articles by this author AMMON B. PECK More articles by this author KAREN J. BYER More articles by this author SAEED R. KHAN More articles by this author Expand All Advertisement PDF downloadLoading ..." @default.
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• W2010457940 title "EXPRESSION OF BIKUNIN mRNA IN RENAL EPITHELIAL CELLS AFTER OXALATE EXPOSURE" @default.
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