FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010459548> ?p ?o ?g. }

• W2010459548 endingPage "80" @default.
• W2010459548 startingPage "80" @default.
• W2010459548 abstract "The application of flow cytometry on telomeric length determination was initially described by Hultdin et al. (1) and Rufer et al. (2) in 1998. Since then similar techniques have been used for the study of telomere length regulation in immunobiology(3-5), transplant biology (6, 7), and hematological disorders (8, 9). Recently, we published a paper reporting the validation and development of quantitative flow cytometric technique in our institute (10). In their letter, Roos and Hultdin emphasised on the need of appropriate internal control and the importance to consider the DNA index (DI) of each sample for correct interpretation of telomere length. We agree fully with the idea of Roos and Hultdin. Their additional information on MOLT-4 actually matched with our description. We have demonstrated that by taking into account the tetraploid nature of MOLT-4 cells, we were able to explain why the Flow FISH fluorescence value obtained was doubled that predicted by our “conversion” curve. Even though K562 has been described as a diploid cell line from our source (American Type Cell Culture), a “correction factor” of 2.32 would be needed for the Flow FISH fluorescence value to be moved back on the “conversion” curve. Even if our Flow FISH fluorescence value for K562 is compensated by the DI provided by Roos and Hultin (DI = 1.46), it is impossible to explain the high fluorescence value obtained by Flow FISH. The correction of Flow FISH fluorescence by DI has not been described in other groups mainly because normal cells were being studied. We agree that DNA analysis must be included in study of true aneuploid and polyploid cells. With more recent development, it is possible to analyse the cell surface markers and telomere length simultaneously by flow cytometry (4, 11). We are looking forward to more knowledge gained on the regulation of telomere length in various systems." @default.
• W2010459548 created "2016-06-24" @default.
• W2010459548 creator A5027076346 @default.
• W2010459548 creator A5075713847 @default.
• W2010459548 date "2001-01-01" @default.
• W2010459548 modified "2023-10-11" @default.
• W2010459548 title "DNA index and Q Flow FISH measurement of telomere length" @default.
• W2010459548 cites W1494739393 @default.
• W2010459548 cites W1589551152 @default.
• W2010459548 cites W1985130197 @default.
• W2010459548 cites W2006660996 @default.
• W2010459548 cites W2027190304 @default.
• W2010459548 cites W2034960773 @default.
• W2010459548 cites W2048182519 @default.
• W2010459548 cites W2052260390 @default.
• W2010459548 cites W2064651531 @default.
• W2010459548 cites W2074654098 @default.
• W2010459548 cites W2182386828 @default.
• W2010459548 doi "https://doi.org/10.1002/1097-0320(20010901)45:1<80::aid-cyto1148>3.0.co;2-c" @default.
• W2010459548 hasPublicationYear "2001" @default.
• W2010459548 type Work @default.
• W2010459548 sameAs 2010459548 @default.
• W2010459548 citedByCount "5" @default.
• W2010459548 crossrefType "journal-article" @default.
• W2010459548 hasAuthorship W2010459548A5027076346 @default.
• W2010459548 hasAuthorship W2010459548A5075713847 @default.
• W2010459548 hasConcept C104317684 @default.
• W2010459548 hasConcept C121332964 @default.
• W2010459548 hasConcept C143191323 @default.
• W2010459548 hasConcept C153911025 @default.
• W2010459548 hasConcept C177336024 @default.
• W2010459548 hasConcept C2909208804 @default.
• W2010459548 hasConcept C505870484 @default.
• W2010459548 hasConcept C54355233 @default.
• W2010459548 hasConcept C552990157 @default.
• W2010459548 hasConcept C553184892 @default.
• W2010459548 hasConcept C62520636 @default.
• W2010459548 hasConcept C86803240 @default.
• W2010459548 hasConcept C91881484 @default.
• W2010459548 hasConceptScore W2010459548C104317684 @default.
• W2010459548 hasConceptScore W2010459548C121332964 @default.
• W2010459548 hasConceptScore W2010459548C143191323 @default.
• W2010459548 hasConceptScore W2010459548C153911025 @default.
• W2010459548 hasConceptScore W2010459548C177336024 @default.
• W2010459548 hasConceptScore W2010459548C2909208804 @default.
• W2010459548 hasConceptScore W2010459548C505870484 @default.
• W2010459548 hasConceptScore W2010459548C54355233 @default.
• W2010459548 hasConceptScore W2010459548C552990157 @default.
• W2010459548 hasConceptScore W2010459548C553184892 @default.
• W2010459548 hasConceptScore W2010459548C62520636 @default.
• W2010459548 hasConceptScore W2010459548C86803240 @default.
• W2010459548 hasConceptScore W2010459548C91881484 @default.
• W2010459548 hasIssue "1" @default.
• W2010459548 hasLocation W20104595481 @default.
• W2010459548 hasOpenAccess W2010459548 @default.
• W2010459548 hasPrimaryLocation W20104595481 @default.
• W2010459548 hasRelatedWork W1540456588 @default.
• W2010459548 hasRelatedWork W2005903392 @default.
• W2010459548 hasRelatedWork W2014531949 @default.
• W2010459548 hasRelatedWork W2045637853 @default.
• W2010459548 hasRelatedWork W2096760656 @default.
• W2010459548 hasRelatedWork W2103156000 @default.
• W2010459548 hasRelatedWork W2175325490 @default.
• W2010459548 hasRelatedWork W2362612357 @default.
• W2010459548 hasRelatedWork W2417511905 @default.
• W2010459548 hasRelatedWork W4295073628 @default.
• W2010459548 hasVolume "45" @default.
• W2010459548 isParatext "false" @default.
• W2010459548 isRetracted "false" @default.
• W2010459548 magId "2010459548" @default.
• W2010459548 workType "article" @default.