FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010495596> ?p ?o ?g. }

• W2010495596 abstract "In recent years, it has become apparent that genomic instability is tightly related to many developmental disorders, cancers, and aging. Given that stem cells are responsible for ensuring tissue homeostasis and repair throughout life, it is reasonable to hypothesize that the stem cell population is critical for preserving genomic integrity of tissues. Therefore, significant interest has arisen in assessing the impact of endogenous and environmental factors on genomic integrity in stem cells and their progeny, aiming to understand the etiology of stem-cell based diseases. LacI transgenic mice carry a recoverable λ phage vector encoding the LacI reporter system, in which the LacI gene serves as the mutation reporter. The result of a mutated LacI gene is the production of β-galactosidase that cleaves a chromogenic substrate, turning it blue. The LacI reporter system is carried in all cells, including stem/progenitor cells and can easily be recovered and used to subsequently infect E. coli. After incubating infected E. coli on agarose that contains the correct substrate, plaques can be scored; blue plaques indicate a mutant LacI gene, while clear plaques harbor wild-type. The frequency of blue (among clear) plaques indicates the mutant frequency in the original cell population the DNA was extracted from. Sequencing the mutant LacI gene will show the location of the mutations in the gene and the type of mutation. The LacI transgenic mouse model is well-established as an in vivo mutagenesis assay. Moreover, the mice and the reagents for the assay are commercially available. Here we describe in detail how this model can be adapted to measure the frequency of spontaneously occurring DNA mutants in stem cell-enriched Lin(-)IL7R(-)Sca-1(+)cKit(++)(LSK) cells and other subpopulations of the hematopoietic system." @default.
• W2010495596 created "2016-06-24" @default.
• W2010495596 creator A5000727084 @default.
• W2010495596 creator A5030514536 @default.
• W2010495596 creator A5033087897 @default.
• W2010495596 creator A5033261373 @default.
• W2010495596 creator A5062264266 @default.
• W2010495596 creator A5069136446 @default.
• W2010495596 creator A5082141776 @default.
• W2010495596 creator A5090334867 @default.
• W2010495596 date "2014-02-24" @default.
• W2010495596 modified "2023-09-25" @default.
• W2010495596 title "Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells" @default.
• W2010495596 cites W1552247668 @default.
• W2010495596 cites W1979413007 @default.
• W2010495596 cites W1987450771 @default.
• W2010495596 cites W1991029393 @default.
• W2010495596 cites W1992803910 @default.
• W2010495596 cites W1997425941 @default.
• W2010495596 cites W2007968891 @default.
• W2010495596 cites W2024191382 @default.
• W2010495596 cites W2028804912 @default.
• W2010495596 cites W2030658954 @default.
• W2010495596 cites W2030686053 @default.
• W2010495596 cites W2034604700 @default.
• W2010495596 cites W2041724441 @default.
• W2010495596 cites W2042419699 @default.
• W2010495596 cites W2048122201 @default.
• W2010495596 cites W2048281064 @default.
• W2010495596 cites W2049544435 @default.
• W2010495596 cites W2057868602 @default.
• W2010495596 cites W2063062036 @default.
• W2010495596 cites W2067466498 @default.
• W2010495596 cites W2077330278 @default.
• W2010495596 cites W2080337468 @default.
• W2010495596 cites W2083632015 @default.
• W2010495596 cites W2090786449 @default.
• W2010495596 cites W2091156312 @default.
• W2010495596 cites W2091768740 @default.
• W2010495596 cites W2093170540 @default.
• W2010495596 cites W2093845572 @default.
• W2010495596 cites W2097879198 @default.
• W2010495596 cites W2108244937 @default.
• W2010495596 cites W2120634098 @default.
• W2010495596 cites W2128149336 @default.
• W2010495596 cites W2131910049 @default.
• W2010495596 cites W2144671805 @default.
• W2010495596 cites W2149843008 @default.
• W2010495596 cites W2157830903 @default.
• W2010495596 cites W2169238431 @default.
• W2010495596 cites W2263307479 @default.
• W2010495596 cites W2344820924 @default.
• W2010495596 cites W2414005403 @default.
• W2010495596 doi "https://doi.org/10.3791/50752" @default.
• W2010495596 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4130634" @default.
• W2010495596 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24637843" @default.
• W2010495596 hasPublicationYear "2014" @default.
• W2010495596 type Work @default.
• W2010495596 sameAs 2010495596 @default.
• W2010495596 citedByCount "0" @default.
• W2010495596 crossrefType "journal-article" @default.
• W2010495596 hasAuthorship W2010495596A5000727084 @default.
• W2010495596 hasAuthorship W2010495596A5030514536 @default.
• W2010495596 hasAuthorship W2010495596A5033087897 @default.
• W2010495596 hasAuthorship W2010495596A5033261373 @default.
• W2010495596 hasAuthorship W2010495596A5062264266 @default.
• W2010495596 hasAuthorship W2010495596A5069136446 @default.
• W2010495596 hasAuthorship W2010495596A5082141776 @default.
• W2010495596 hasAuthorship W2010495596A5090334867 @default.
• W2010495596 hasBestOaLocation W20104955962 @default.
• W2010495596 hasConcept C102230213 @default.
• W2010495596 hasConcept C104317684 @default.
• W2010495596 hasConcept C113629113 @default.
• W2010495596 hasConcept C143065580 @default.
• W2010495596 hasConcept C144024400 @default.
• W2010495596 hasConcept C149923435 @default.
• W2010495596 hasConcept C150194340 @default.
• W2010495596 hasConcept C153911025 @default.
• W2010495596 hasConcept C161733203 @default.
• W2010495596 hasConcept C16318435 @default.
• W2010495596 hasConcept C170344550 @default.
• W2010495596 hasConcept C17757408 @default.
• W2010495596 hasConcept C201750760 @default.
• W2010495596 hasConcept C28328180 @default.
• W2010495596 hasConcept C2908647359 @default.
• W2010495596 hasConcept C501734568 @default.
• W2010495596 hasConcept C54355233 @default.
• W2010495596 hasConcept C86803240 @default.
• W2010495596 hasConcept C95444343 @default.
• W2010495596 hasConceptScore W2010495596C102230213 @default.
• W2010495596 hasConceptScore W2010495596C104317684 @default.
• W2010495596 hasConceptScore W2010495596C113629113 @default.
• W2010495596 hasConceptScore W2010495596C143065580 @default.
• W2010495596 hasConceptScore W2010495596C144024400 @default.
• W2010495596 hasConceptScore W2010495596C149923435 @default.
• W2010495596 hasConceptScore W2010495596C150194340 @default.
• W2010495596 hasConceptScore W2010495596C153911025 @default.
• W2010495596 hasConceptScore W2010495596C161733203 @default.
• W2010495596 hasConceptScore W2010495596C16318435 @default.
• W2010495596 hasConceptScore W2010495596C170344550 @default.

• next