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• W2010500907 abstract "The survival of cells subjected to cooling at sub-zero temperature is of paramount concern in cryobiology. The susceptibility of cells to cryopreservation processes, especially freeze-thawing, stimulated considerable interest in better understanding the mechanisms leading to cell injury and inactivation. In this study, we assessed the viability of cells subjected to cold stress, through long-term supercooling experiments, versus freeze-thawing stress. The viability of Escherichia coli, Saccharomyces cerevisiae, and leukemia cells were assessed over time. Supercooled conditions were maintained for 71 days at −10°C, and for 4 h at −15°C, and −20°C, without additives or emulsification. Results showed that cells could be inactivated by the only action of sub-zero temperature, that is, without any water crystallization. The loss of cell viability upon exposure to sub-zero temperatures is suggested to be caused by exposure to cold shock which induced membrane damage. During holding time in the supercooled state, elevated membrane permeability results in uncontrolled mass transfer to and from the cell maintained at cold conditions and thus leads to a loss of viability. With water crystallization, cells shrink suddenly and thus are exposed to cold osmotic shock, which is suggested to induce abrupt loss of cell viability. During holding time in the frozen state, cells remain suspended in the residual unfrozen fraction of the liquid and are exposed to cold stress that would cause membrane damage and loss of viability over time. However, the severity of such a stress seems to be moderated by the cell type and the increased solute concentration in the unfrozen fraction of the cell suspension. Biotechnol. Bioeng. © 2008 Wiley Periodicals, Inc." @default.
• W2010500907 created "2016-06-24" @default.
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• W2010500907 date "2008-12-15" @default.
• W2010500907 modified "2023-10-17" @default.
• W2010500907 title "Cell inactivation and membrane damage after long-term treatments at sub-zero temperature in the supercooled and frozen states" @default.
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• W2010500907 doi "https://doi.org/10.1002/bit.21981" @default.
• W2010500907 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18814283" @default.
• W2010500907 hasPublicationYear "2008" @default.
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