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• W2010505745 abstract "Single-cell analysis is a powerful method to assess the heterogeneity among individual cells, enabling the identification of very rare cells with properties that differ from those of the majority. In this Methods Article, we describe the use of a large-scale femtoliter droplet array to enclose, isolate, and analyze individual bacterial cells. As a first example, we describe the single-cell detection of drug-tolerant persisters of Pseudomonas aeruginosa treated with the antibiotic carbenicillin. As a second example, this method was applied to the single-cell evaluation of drug efflux activity, which causes acquired antibiotic resistance of bacteria. The activity of the MexAB-OprM multidrug efflux pump system from Pseudomonas aeruginosa was expressed in Escherichia coli and the effect of an inhibitor D13-9001 were assessed at the single cell level." @default.
• W2010505745 created "2016-06-24" @default.
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• W2010505745 date "2013-01-01" @default.
• W2010505745 modified "2023-09-30" @default.
• W2010505745 title "Design of a large-scale femtoliter droplet array for single-cell analysis of drug-tolerant and drug-resistant bacteria" @default.
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• W2010505745 doi "https://doi.org/10.3389/fmicb.2013.00300" @default.
• W2010505745 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3790107" @default.
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