Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010505945> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2010505945 endingPage "127" @default.
- W2010505945 startingPage "119" @default.
- W2010505945 abstract "A glucose dehydrogenase (d-glucose:NAD(P)+ 1-oxidoreductase, EC 1.1.1.118(9)) was purified over 1100-fold, to near homogeneity, from Nostoc sp. strain MAC cells. The general enzymatic properties of the Nostoc enzyme were similar to those of the NAD(P)-dependent glucose dehydrogenase isolated earlier from facultatively heterotrophic cyanobacteria, heterotrophic bacteria and liver cells. In this work, we show that Nostoc glucose dehydrogenase can be modulated by various redox systems. Reducing agents such as dithiothreitol, cysteine and 2-mercaptoethanol decreased the activity of the enzyme. Reduced glutathione and ascorbic acid exerted only slight influence. The oxido-reductive modulation proved to be reversible. Treatment of the reduced glucose dehydrogenase with oxidants such as H2O2, Fe3+-EDTA and a Fe3+-chelate (Udvardy, J.; Borbély, Gy., Juhász, A.; and Farkas, G.L. (1984) FEBS Lett. 172, 11–16) reversed the process of deactivation. The range of the reductive deactivation depended on pH, being the most effective in the alkaline range. Addition of 10 mM Mg2+ and Ca2+ in the cuvette to the reduced form of glucose dehydrogenase, however, reactivated the enzyme. These cations had little effect on the oxidized enzyme. Mg2+ and Ca2+ changed the kinetic parameters (Km and Vmax) of the reduced, but not of the oxidized form of the Nostoc glucose dehydrogenase. Redox modulation was not accompanied by any change in the apparent molecular mass (110–120 kDa)." @default.
- W2010505945 created "2016-06-24" @default.
- W2010505945 creator A5030936225 @default.
- W2010505945 creator A5037366335 @default.
- W2010505945 creator A5047305526 @default.
- W2010505945 creator A5088991914 @default.
- W2010505945 date "1987-11-01" @default.
- W2010505945 modified "2023-09-27" @default.
- W2010505945 title "Redox regulation of glucose dehydrogenase from cells of the facultatively heterotrophic cyanobacterium Nostoc sp. strain MAC" @default.
- W2010505945 cites W1489744792 @default.
- W2010505945 cites W1531076517 @default.
- W2010505945 cites W1549339526 @default.
- W2010505945 cites W1602814448 @default.
- W2010505945 cites W167158724 @default.
- W2010505945 cites W1775749144 @default.
- W2010505945 cites W1966103338 @default.
- W2010505945 cites W1976912793 @default.
- W2010505945 cites W1988064189 @default.
- W2010505945 cites W1994496688 @default.
- W2010505945 cites W2007927027 @default.
- W2010505945 cites W2014336412 @default.
- W2010505945 cites W2015345433 @default.
- W2010505945 cites W2015916978 @default.
- W2010505945 cites W2018104391 @default.
- W2010505945 cites W2023345403 @default.
- W2010505945 cites W2032820794 @default.
- W2010505945 cites W2038022806 @default.
- W2010505945 cites W2044436199 @default.
- W2010505945 cites W2061256376 @default.
- W2010505945 cites W2064804975 @default.
- W2010505945 cites W2074241139 @default.
- W2010505945 cites W2081251927 @default.
- W2010505945 cites W2113903877 @default.
- W2010505945 cites W2114293183 @default.
- W2010505945 cites W2333317961 @default.
- W2010505945 cites W2410773404 @default.
- W2010505945 cites W2414444155 @default.
- W2010505945 cites W3127442976 @default.
- W2010505945 cites W2061739804 @default.
- W2010505945 doi "https://doi.org/10.1016/0167-4838(87)90218-4" @default.
- W2010505945 hasPublicationYear "1987" @default.
- W2010505945 type Work @default.
- W2010505945 sameAs 2010505945 @default.
- W2010505945 citedByCount "3" @default.
- W2010505945 crossrefType "journal-article" @default.
- W2010505945 hasAuthorship W2010505945A5030936225 @default.
- W2010505945 hasAuthorship W2010505945A5037366335 @default.
- W2010505945 hasAuthorship W2010505945A5047305526 @default.
- W2010505945 hasAuthorship W2010505945A5088991914 @default.
- W2010505945 hasConcept C179104552 @default.
- W2010505945 hasConcept C181199279 @default.
- W2010505945 hasConcept C185592680 @default.
- W2010505945 hasConcept C2776317432 @default.
- W2010505945 hasConcept C2777824588 @default.
- W2010505945 hasConcept C2779268744 @default.
- W2010505945 hasConcept C2779465136 @default.
- W2010505945 hasConcept C2779669040 @default.
- W2010505945 hasConcept C523546767 @default.
- W2010505945 hasConcept C54355233 @default.
- W2010505945 hasConcept C55493867 @default.
- W2010505945 hasConcept C55904794 @default.
- W2010505945 hasConcept C75520062 @default.
- W2010505945 hasConcept C86803240 @default.
- W2010505945 hasConceptScore W2010505945C179104552 @default.
- W2010505945 hasConceptScore W2010505945C181199279 @default.
- W2010505945 hasConceptScore W2010505945C185592680 @default.
- W2010505945 hasConceptScore W2010505945C2776317432 @default.
- W2010505945 hasConceptScore W2010505945C2777824588 @default.
- W2010505945 hasConceptScore W2010505945C2779268744 @default.
- W2010505945 hasConceptScore W2010505945C2779465136 @default.
- W2010505945 hasConceptScore W2010505945C2779669040 @default.
- W2010505945 hasConceptScore W2010505945C523546767 @default.
- W2010505945 hasConceptScore W2010505945C54355233 @default.
- W2010505945 hasConceptScore W2010505945C55493867 @default.
- W2010505945 hasConceptScore W2010505945C55904794 @default.
- W2010505945 hasConceptScore W2010505945C75520062 @default.
- W2010505945 hasConceptScore W2010505945C86803240 @default.
- W2010505945 hasIssue "1" @default.
- W2010505945 hasLocation W20105059451 @default.
- W2010505945 hasOpenAccess W2010505945 @default.
- W2010505945 hasPrimaryLocation W20105059451 @default.
- W2010505945 hasRelatedWork W1536243931 @default.
- W2010505945 hasRelatedWork W1545314684 @default.
- W2010505945 hasRelatedWork W1656364975 @default.
- W2010505945 hasRelatedWork W1953994264 @default.
- W2010505945 hasRelatedWork W2000986031 @default.
- W2010505945 hasRelatedWork W2001274806 @default.
- W2010505945 hasRelatedWork W2071151402 @default.
- W2010505945 hasRelatedWork W2075537445 @default.
- W2010505945 hasRelatedWork W2154133864 @default.
- W2010505945 hasRelatedWork W9978199 @default.
- W2010505945 hasVolume "916" @default.
- W2010505945 isParatext "false" @default.
- W2010505945 isRetracted "false" @default.
- W2010505945 magId "2010505945" @default.
- W2010505945 workType "article" @default.