Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010512829> ?p ?o ?g. }
- W2010512829 endingPage "483" @default.
- W2010512829 startingPage "473" @default.
- W2010512829 abstract "Hyperfluorescent intensity maxima during protein unfolding titrations are often taken as a sign for a thermodynamic folding intermediate. Here we explore another possibility: that hyperfluorescence could be the signature of a pretransition conformationally loosened native state. To model such native states, we study mutants of a fluorescent ubiquitin variant, placing cavities at various distances from the tryptophan fluorophore. We examine the correlation between protein flexibility and enhanced fluorescence intensity by using circular dichroism, fluorescence intensity unfolding titrations, fluorescence anisotropy measurements, and molecular dynamics. Based on experiment and simulation, we propose a simple model for hyperfluorescence in terms of static and dynamic conformational properties of the native state during unfolding. Apomyoglobin denaturant unfolding and phosphoglycerate kinase cold denaturation are discussed as examples. Our results do not preclude the existence of thermodynamic intermediates but do raise caution that by itself, hyperfluorescence during unfolding titrations is not conclusive proof of thermodynamic folding intermediates." @default.
- W2010512829 created "2016-06-24" @default.
- W2010512829 creator A5009810193 @default.
- W2010512829 creator A5016032737 @default.
- W2010512829 creator A5029996336 @default.
- W2010512829 creator A5042251445 @default.
- W2010512829 creator A5090064457 @default.
- W2010512829 date "2002-07-01" @default.
- W2010512829 modified "2023-10-17" @default.
- W2010512829 title "What Causes Hyperfluorescence: Folding Intermediates or Conformationally Flexible Native States?" @default.
- W2010512829 cites W1493864303 @default.
- W2010512829 cites W1496359087 @default.
- W2010512829 cites W1593429702 @default.
- W2010512829 cites W1964610252 @default.
- W2010512829 cites W1965149799 @default.
- W2010512829 cites W1978167946 @default.
- W2010512829 cites W1985011933 @default.
- W2010512829 cites W1987208202 @default.
- W2010512829 cites W2008165421 @default.
- W2010512829 cites W2011764481 @default.
- W2010512829 cites W2014303929 @default.
- W2010512829 cites W2024421359 @default.
- W2010512829 cites W2026650105 @default.
- W2010512829 cites W2029667189 @default.
- W2010512829 cites W2034278689 @default.
- W2010512829 cites W2036659800 @default.
- W2010512829 cites W2041721370 @default.
- W2010512829 cites W2042360874 @default.
- W2010512829 cites W2047520644 @default.
- W2010512829 cites W2051673588 @default.
- W2010512829 cites W2052586436 @default.
- W2010512829 cites W2054342921 @default.
- W2010512829 cites W2064405226 @default.
- W2010512829 cites W2069249321 @default.
- W2010512829 cites W2069479024 @default.
- W2010512829 cites W2074970710 @default.
- W2010512829 cites W2074991560 @default.
- W2010512829 cites W2076945171 @default.
- W2010512829 cites W2078040293 @default.
- W2010512829 cites W2080449760 @default.
- W2010512829 cites W2081332395 @default.
- W2010512829 cites W2084280203 @default.
- W2010512829 cites W2087643633 @default.
- W2010512829 cites W2094795801 @default.
- W2010512829 cites W2103815459 @default.
- W2010512829 cites W2110536097 @default.
- W2010512829 cites W2119586900 @default.
- W2010512829 cites W2131643591 @default.
- W2010512829 cites W2139327615 @default.
- W2010512829 cites W2156012420 @default.
- W2010512829 cites W2162166182 @default.
- W2010512829 cites W2167241357 @default.
- W2010512829 cites W2905684965 @default.
- W2010512829 doi "https://doi.org/10.1016/s0006-3495(02)75183-7" @default.
- W2010512829 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1302161" @default.
- W2010512829 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12080134" @default.
- W2010512829 hasPublicationYear "2002" @default.
- W2010512829 type Work @default.
- W2010512829 sameAs 2010512829 @default.
- W2010512829 citedByCount "53" @default.
- W2010512829 countsByYear W20105128292012 @default.
- W2010512829 countsByYear W20105128292013 @default.
- W2010512829 countsByYear W20105128292014 @default.
- W2010512829 countsByYear W20105128292015 @default.
- W2010512829 countsByYear W20105128292016 @default.
- W2010512829 countsByYear W20105128292018 @default.
- W2010512829 countsByYear W20105128292019 @default.
- W2010512829 countsByYear W20105128292020 @default.
- W2010512829 countsByYear W20105128292021 @default.
- W2010512829 countsByYear W20105128292022 @default.
- W2010512829 countsByYear W20105128292023 @default.
- W2010512829 crossrefType "journal-article" @default.
- W2010512829 hasAuthorship W2010512829A5009810193 @default.
- W2010512829 hasAuthorship W2010512829A5016032737 @default.
- W2010512829 hasAuthorship W2010512829A5029996336 @default.
- W2010512829 hasAuthorship W2010512829A5042251445 @default.
- W2010512829 hasAuthorship W2010512829A5090064457 @default.
- W2010512829 hasBestOaLocation W20105128291 @default.
- W2010512829 hasConcept C119599485 @default.
- W2010512829 hasConcept C121332964 @default.
- W2010512829 hasConcept C127413603 @default.
- W2010512829 hasConcept C138885662 @default.
- W2010512829 hasConcept C13965031 @default.
- W2010512829 hasConcept C147597530 @default.
- W2010512829 hasConcept C147789679 @default.
- W2010512829 hasConcept C159467904 @default.
- W2010512829 hasConcept C184866935 @default.
- W2010512829 hasConcept C185592680 @default.
- W2010512829 hasConcept C204328495 @default.
- W2010512829 hasConcept C27206212 @default.
- W2010512829 hasConcept C2776545253 @default.
- W2010512829 hasConcept C2776923230 @default.
- W2010512829 hasConcept C2778617687 @default.
- W2010512829 hasConcept C2780702060 @default.
- W2010512829 hasConcept C55493867 @default.
- W2010512829 hasConcept C59593255 @default.
- W2010512829 hasConcept C62520636 @default.
- W2010512829 hasConcept C8010536 @default.