FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010533241> ?p ?o ?g. }

• W2010533241 endingPage "553" @default.
• W2010533241 startingPage "531" @default.
• W2010533241 abstract "There are great demands on the stability, expression yield and resistance to aggregation of antibody fragments. To untangle intrinsic domain effects from domain interactions, we present first a systematic evaluation of the isolated human immunoglobulin variable heavy (V(H)) and light (V(L)) germline family consensus domains and then a systematic series of V(H)-V(L) combinations in the scFv format. The constructs were evaluated in terms of their expression behavior, oligomeric state in solution and denaturant-induced unfolding equilibria under non-reducing conditions. The seven V(H) and seven V(L) domains represent the consensus sequences of the major human germline subclasses, derived from the Human Combinatorial Antibody Library (HuCAL). The isolated V(H) and V(L) domains with the highest thermodynamic stability and yield of soluble protein were V(H)3 and V(kappa)3, respectively. Similar measurements on all domain combinations in scFv fragments allowed the scFv fragments to be classified according to thermodynamic stability and in vivo folding yield. The scFv fragments containing the variable domain combinations H3kappa3, H1bkappa3, H5kappa3 and H3kappa1 show superior properties concerning yield and stability. Domain interactions diminish the intrinsic differences of the domains. ScFv fragments containing V(lambda) domains show high levels of stability, even though V(lambda) domains are surprisingly unstable by themselves. This is due to a strong interaction with the V(H) domain and depends on the amino acid sequence of the CDR-L3. On the basis of these analyses and model structures, we suggest possibilities for further improvement of the biophysical properties of individual frameworks and give recommendations for library design." @default.
• W2010533241 created "2016-06-24" @default.
• W2010533241 creator A5029902815 @default.
• W2010533241 creator A5046786993 @default.
• W2010533241 creator A5064676018 @default.
• W2010533241 creator A5083658123 @default.
• W2010533241 date "2003-01-01" @default.
• W2010533241 modified "2023-10-14" @default.
• W2010533241 title "Biophysical Properties of Human Antibody Variable Domains" @default.
• W2010533241 cites W1533325200 @default.
• W2010533241 cites W1606431180 @default.
• W2010533241 cites W1963994022 @default.
• W2010533241 cites W1968704981 @default.
• W2010533241 cites W1970138467 @default.
• W2010533241 cites W1970887331 @default.
• W2010533241 cites W1976784576 @default.
• W2010533241 cites W1980260012 @default.
• W2010533241 cites W1984618618 @default.
• W2010533241 cites W1986213229 @default.
• W2010533241 cites W1989233913 @default.
• W2010533241 cites W1996192954 @default.
• W2010533241 cites W1997830689 @default.
• W2010533241 cites W2002195659 @default.
• W2010533241 cites W2006174096 @default.
• W2010533241 cites W2006174330 @default.
• W2010533241 cites W2007930265 @default.
• W2010533241 cites W2009179035 @default.
• W2010533241 cites W2020972820 @default.
• W2010533241 cites W2022228265 @default.
• W2010533241 cites W2027224583 @default.
• W2010533241 cites W2048452070 @default.
• W2010533241 cites W2050602939 @default.
• W2010533241 cites W2054774755 @default.
• W2010533241 cites W2055152097 @default.
• W2010533241 cites W2056435485 @default.
• W2010533241 cites W2059445608 @default.
• W2010533241 cites W2062653170 @default.
• W2010533241 cites W2070011490 @default.
• W2010533241 cites W2072948213 @default.
• W2010533241 cites W2073517578 @default.
• W2010533241 cites W2073551759 @default.
• W2010533241 cites W2078070662 @default.
• W2010533241 cites W2085881249 @default.
• W2010533241 cites W2108353209 @default.
• W2010533241 cites W2109008135 @default.
• W2010533241 cites W2110340689 @default.
• W2010533241 cites W2112922048 @default.
• W2010533241 cites W2115970730 @default.
• W2010533241 cites W2116576596 @default.
• W2010533241 cites W2117747873 @default.
• W2010533241 cites W2118817949 @default.
• W2010533241 cites W2118888416 @default.
• W2010533241 cites W2140393594 @default.
• W2010533241 cites W2144886557 @default.
• W2010533241 cites W2151838728 @default.
• W2010533241 cites W2152135678 @default.
• W2010533241 cites W2153644085 @default.
• W2010533241 cites W2155018679 @default.
• W2010533241 cites W2155184368 @default.
• W2010533241 cites W2156037313 @default.
• W2010533241 cites W2157194928 @default.
• W2010533241 cites W2160034474 @default.
• W2010533241 cites W2160988970 @default.
• W2010533241 doi "https://doi.org/10.1016/s0022-2836(02)01237-8" @default.
• W2010533241 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12498801" @default.
• W2010533241 hasPublicationYear "2003" @default.
• W2010533241 type Work @default.
• W2010533241 sameAs 2010533241 @default.
• W2010533241 citedByCount "308" @default.
• W2010533241 countsByYear W20105332412012 @default.
• W2010533241 countsByYear W20105332412013 @default.
• W2010533241 countsByYear W20105332412014 @default.
• W2010533241 countsByYear W20105332412015 @default.
• W2010533241 countsByYear W20105332412016 @default.
• W2010533241 countsByYear W20105332412017 @default.
• W2010533241 countsByYear W20105332412018 @default.
• W2010533241 countsByYear W20105332412019 @default.
• W2010533241 countsByYear W20105332412020 @default.
• W2010533241 countsByYear W20105332412021 @default.
• W2010533241 countsByYear W20105332412022 @default.
• W2010533241 countsByYear W20105332412023 @default.
• W2010533241 crossrefType "journal-article" @default.
• W2010533241 hasAuthorship W2010533241A5029902815 @default.
• W2010533241 hasAuthorship W2010533241A5046786993 @default.
• W2010533241 hasAuthorship W2010533241A5064676018 @default.
• W2010533241 hasAuthorship W2010533241A5083658123 @default.
• W2010533241 hasConcept C119599485 @default.
• W2010533241 hasConcept C121332964 @default.
• W2010533241 hasConcept C12554922 @default.
• W2010533241 hasConcept C127413603 @default.
• W2010533241 hasConcept C134121241 @default.
• W2010533241 hasConcept C134306372 @default.
• W2010533241 hasConcept C185592680 @default.
• W2010533241 hasConcept C204328495 @default.
• W2010533241 hasConcept C2776545253 @default.
• W2010533241 hasConcept C33923547 @default.
• W2010533241 hasConcept C36503486 @default.
• W2010533241 hasConcept C515207424 @default.

• next