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- W2010547450 abstract "A sensitive GC-ECD assay has been developed for the simultaneous determination of midazolam (I) and its α-hydroxy metabolite (II) in plasma. The assay involves extraction of both compounds into ether at alkaline pH (pH 12), followed by silylation of the α-hydroxy metabolite with N,O-bis(trimethylsilyl) trifluoroacetamide (BSTFA). On extracting 0.5 ml of plasma, the sensitivity limits are 4ng/ml for I and 3ng/ml for II. If present, the minor urinary metabolites, the 4-hydroxy (III) and the α,4-dihydroxy compound (IV), can also be determined by this method." @default.
- W2010547450 created "2016-06-24" @default.
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- W2010547450 date "1981-06-01" @default.
- W2010547450 modified "2023-10-16" @default.
- W2010547450 title "Determination of midazolam and its α-hydroxy metabolite in plasma by gas chromatography with electron capture detection" @default.
- W2010547450 cites W2049691579 @default.
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- W2010547450 doi "https://doi.org/10.1002/jhrc.1240040604" @default.
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