FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010599804> ?p ?o ?g. }

• W2010599804 endingPage "185" @default.
• W2010599804 startingPage "178" @default.
• W2010599804 abstract "The nature of the primary lesions suffered by cells during freezing and thawing is unclear, although the plasma membrane is often considered the primary site for freezing injury. This study was designed to investigate the nature of damage immediately after thawing, by monitoring several functional tests of the cell and the plasma membrane. Hamster fibroblasts, human lymphocytes, and human granulocytes were subjected to a graded freeze-thaw stress in the absence of cryoprotective compound by cooling at -1 degree C/min to a temperature between -10 and -40 degrees C, and then were either warmed directly in water at 37 degrees C or cooled rapidly to -196 degrees C before rapid warming. Mitochondrial function in the cells was then assessed using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT), fluorescein diacetate (FDA), colony growth, and osmometric response in a hypertonic solution. Cells behaved as osmometers after cooling at -1 degree C/min to low temperatures at which there were no responses measured by other assays, indicating that the plasma membrane is not a primary site for injury sustained during slow cooling. These results also indicate that the FDA test does not measure membrane integrity, but reflects the permeability of the channels through which fluorescein leaves the cells. Fewer cells could respond osmotically after cooling under conditions where intracellular freezing was likely, implying that the plasma membrane is directly damaged by the conditions leading to intracellular freezing. A general model of freezing injury to nucleated mammalian cells is proposed in which disruption of the lysosomes constitutes the primary lesion in cells cooled under conditions where the cells are dehydrated at low temperatures." @default.
• W2010599804 created "2016-06-24" @default.
• W2010599804 creator A5006075752 @default.
• W2010599804 creator A5020416877 @default.
• W2010599804 creator A5063025651 @default.
• W2010599804 date "1988-06-01" @default.
• W2010599804 modified "2023-10-17" @default.
• W2010599804 title "Manifestations of cell damage after freezing and thawing" @default.
• W2010599804 cites W1566271574 @default.
• W2010599804 cites W1970422426 @default.
• W2010599804 cites W1980181948 @default.
• W2010599804 cites W2008071524 @default.
• W2010599804 cites W2027445302 @default.
• W2010599804 cites W2035375234 @default.
• W2010599804 cites W2063238343 @default.
• W2010599804 cites W2067916831 @default.
• W2010599804 cites W2086491525 @default.
• W2010599804 cites W2086955370 @default.
• W2010599804 cites W2089625152 @default.
• W2010599804 cites W2114918609 @default.
• W2010599804 cites W2120629404 @default.
• W2010599804 doi "https://doi.org/10.1016/0011-2240(88)90024-7" @default.
• W2010599804 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3396384" @default.
• W2010599804 hasPublicationYear "1988" @default.
• W2010599804 type Work @default.
• W2010599804 sameAs 2010599804 @default.
• W2010599804 citedByCount "92" @default.
• W2010599804 countsByYear W20105998042012 @default.
• W2010599804 countsByYear W20105998042013 @default.
• W2010599804 countsByYear W20105998042014 @default.
• W2010599804 countsByYear W20105998042015 @default.
• W2010599804 countsByYear W20105998042016 @default.
• W2010599804 countsByYear W20105998042017 @default.
• W2010599804 countsByYear W20105998042018 @default.
• W2010599804 countsByYear W20105998042019 @default.
• W2010599804 countsByYear W20105998042020 @default.
• W2010599804 countsByYear W20105998042021 @default.
• W2010599804 countsByYear W20105998042022 @default.
• W2010599804 countsByYear W20105998042023 @default.
• W2010599804 crossrefType "journal-article" @default.
• W2010599804 hasAuthorship W2010599804A5006075752 @default.
• W2010599804 hasAuthorship W2010599804A5020416877 @default.
• W2010599804 hasAuthorship W2010599804A5063025651 @default.
• W2010599804 hasConcept C117277713 @default.
• W2010599804 hasConcept C121332964 @default.
• W2010599804 hasConcept C12554922 @default.
• W2010599804 hasConcept C127313418 @default.
• W2010599804 hasConcept C153911025 @default.
• W2010599804 hasConcept C16685009 @default.
• W2010599804 hasConcept C185592680 @default.
• W2010599804 hasConcept C2778015030 @default.
• W2010599804 hasConcept C2778575167 @default.
• W2010599804 hasConcept C2992270614 @default.
• W2010599804 hasConcept C2992878202 @default.
• W2010599804 hasConcept C41625074 @default.
• W2010599804 hasConcept C55493867 @default.
• W2010599804 hasConcept C62520636 @default.
• W2010599804 hasConcept C71924100 @default.
• W2010599804 hasConcept C79879829 @default.
• W2010599804 hasConcept C86803240 @default.
• W2010599804 hasConcept C91586092 @default.
• W2010599804 hasConcept C91881484 @default.
• W2010599804 hasConceptScore W2010599804C117277713 @default.
• W2010599804 hasConceptScore W2010599804C121332964 @default.
• W2010599804 hasConceptScore W2010599804C12554922 @default.
• W2010599804 hasConceptScore W2010599804C127313418 @default.
• W2010599804 hasConceptScore W2010599804C153911025 @default.
• W2010599804 hasConceptScore W2010599804C16685009 @default.
• W2010599804 hasConceptScore W2010599804C185592680 @default.
• W2010599804 hasConceptScore W2010599804C2778015030 @default.
• W2010599804 hasConceptScore W2010599804C2778575167 @default.
• W2010599804 hasConceptScore W2010599804C2992270614 @default.
• W2010599804 hasConceptScore W2010599804C2992878202 @default.
• W2010599804 hasConceptScore W2010599804C41625074 @default.
• W2010599804 hasConceptScore W2010599804C55493867 @default.
• W2010599804 hasConceptScore W2010599804C62520636 @default.
• W2010599804 hasConceptScore W2010599804C71924100 @default.
• W2010599804 hasConceptScore W2010599804C79879829 @default.
• W2010599804 hasConceptScore W2010599804C86803240 @default.
• W2010599804 hasConceptScore W2010599804C91586092 @default.
• W2010599804 hasConceptScore W2010599804C91881484 @default.
• W2010599804 hasIssue "3" @default.
• W2010599804 hasLocation W20105998041 @default.
• W2010599804 hasLocation W20105998042 @default.
• W2010599804 hasOpenAccess W2010599804 @default.
• W2010599804 hasPrimaryLocation W20105998041 @default.
• W2010599804 hasRelatedWork W175213470 @default.
• W2010599804 hasRelatedWork W1967866264 @default.
• W2010599804 hasRelatedWork W2010599804 @default.
• W2010599804 hasRelatedWork W2078868720 @default.
• W2010599804 hasRelatedWork W2085862980 @default.
• W2010599804 hasRelatedWork W2086253892 @default.
• W2010599804 hasRelatedWork W2154012993 @default.
• W2010599804 hasRelatedWork W2400849920 @default.
• W2010599804 hasRelatedWork W2410571041 @default.
• W2010599804 hasRelatedWork W4293060754 @default.
• W2010599804 hasVolume "25" @default.
• W2010599804 isParatext "false" @default.

• next