Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010600019> ?p ?o ?g. }
- W2010600019 endingPage "146" @default.
- W2010600019 startingPage "140" @default.
- W2010600019 abstract "Here, the authors present an approach for the simultaneous optogenetic manipulation and recording of neural activity at cellular resolution using two-photon microscopy and apply their strategy in the mouse barrel cortex. We describe an all-optical strategy for simultaneously manipulating and recording the activity of multiple neurons with cellular resolution in vivo. We performed simultaneous two-photon optogenetic activation and calcium imaging by coexpression of a red-shifted opsin and a genetically encoded calcium indicator. A spatial light modulator allows tens of user-selected neurons to be targeted for spatiotemporally precise concurrent optogenetic activation, while simultaneous fast calcium imaging provides high-resolution network-wide readout of the manipulation with negligible optical cross-talk. Proof-of-principle experiments in mouse barrel cortex demonstrate interrogation of the same neuronal population during different behavioral states and targeting of neuronal ensembles based on their functional signature. This approach extends the optogenetic toolkit beyond the specificity obtained with genetic or viral approaches, enabling high-throughput, flexible and long-term optical interrogation of functionally defined neural circuits with single-cell and single-spike resolution in the mouse brain in vivo." @default.
- W2010600019 created "2016-06-24" @default.
- W2010600019 creator A5041323310 @default.
- W2010600019 creator A5078474133 @default.
- W2010600019 creator A5086479155 @default.
- W2010600019 creator A5088723671 @default.
- W2010600019 date "2014-12-22" @default.
- W2010600019 modified "2023-10-15" @default.
- W2010600019 title "Simultaneous all-optical manipulation and recording of neural circuit activity with cellular resolution in vivo" @default.
- W2010600019 cites W1945241997 @default.
- W2010600019 cites W1963937305 @default.
- W2010600019 cites W1970470504 @default.
- W2010600019 cites W1972869628 @default.
- W2010600019 cites W1976485053 @default.
- W2010600019 cites W1994574914 @default.
- W2010600019 cites W1997073648 @default.
- W2010600019 cites W1997247894 @default.
- W2010600019 cites W2002667643 @default.
- W2010600019 cites W2007478889 @default.
- W2010600019 cites W2007494471 @default.
- W2010600019 cites W2011176027 @default.
- W2010600019 cites W2013455564 @default.
- W2010600019 cites W2015520158 @default.
- W2010600019 cites W2023097449 @default.
- W2010600019 cites W2026425363 @default.
- W2010600019 cites W2029932526 @default.
- W2010600019 cites W2038926537 @default.
- W2010600019 cites W2050932531 @default.
- W2010600019 cites W2054797412 @default.
- W2010600019 cites W2061464631 @default.
- W2010600019 cites W2062619493 @default.
- W2010600019 cites W2064716621 @default.
- W2010600019 cites W2067474937 @default.
- W2010600019 cites W2072376983 @default.
- W2010600019 cites W2072652550 @default.
- W2010600019 cites W2074869013 @default.
- W2010600019 cites W2081321639 @default.
- W2010600019 cites W2084771878 @default.
- W2010600019 cites W2085523626 @default.
- W2010600019 cites W2086837225 @default.
- W2010600019 cites W2089278494 @default.
- W2010600019 cites W2097237250 @default.
- W2010600019 cites W2099865122 @default.
- W2010600019 cites W2103655875 @default.
- W2010600019 cites W2109234881 @default.
- W2010600019 cites W2113381337 @default.
- W2010600019 cites W2117425657 @default.
- W2010600019 cites W2124196462 @default.
- W2010600019 cites W2132663329 @default.
- W2010600019 cites W2138997609 @default.
- W2010600019 cites W2145909957 @default.
- W2010600019 cites W2147193337 @default.
- W2010600019 cites W2147249695 @default.
- W2010600019 cites W2150776472 @default.
- W2010600019 cites W2155505956 @default.
- W2010600019 cites W2156814492 @default.
- W2010600019 cites W2160919229 @default.
- W2010600019 cites W2171332611 @default.
- W2010600019 doi "https://doi.org/10.1038/nmeth.3217" @default.
- W2010600019 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4933203" @default.
- W2010600019 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25532138" @default.
- W2010600019 hasPublicationYear "2014" @default.
- W2010600019 type Work @default.
- W2010600019 sameAs 2010600019 @default.
- W2010600019 citedByCount "465" @default.
- W2010600019 countsByYear W20106000192014 @default.
- W2010600019 countsByYear W20106000192015 @default.
- W2010600019 countsByYear W20106000192016 @default.
- W2010600019 countsByYear W20106000192017 @default.
- W2010600019 countsByYear W20106000192018 @default.
- W2010600019 countsByYear W20106000192019 @default.
- W2010600019 countsByYear W20106000192020 @default.
- W2010600019 countsByYear W20106000192021 @default.
- W2010600019 countsByYear W20106000192022 @default.
- W2010600019 countsByYear W20106000192023 @default.
- W2010600019 crossrefType "journal-article" @default.
- W2010600019 hasAuthorship W2010600019A5041323310 @default.
- W2010600019 hasAuthorship W2010600019A5078474133 @default.
- W2010600019 hasAuthorship W2010600019A5086479155 @default.
- W2010600019 hasAuthorship W2010600019A5088723671 @default.
- W2010600019 hasBestOaLocation W20106000192 @default.
- W2010600019 hasConcept C118403218 @default.
- W2010600019 hasConcept C119666444 @default.
- W2010600019 hasConcept C120665830 @default.
- W2010600019 hasConcept C121332964 @default.
- W2010600019 hasConcept C150903083 @default.
- W2010600019 hasConcept C169760540 @default.
- W2010600019 hasConcept C178790620 @default.
- W2010600019 hasConcept C185592680 @default.
- W2010600019 hasConcept C207001950 @default.
- W2010600019 hasConcept C2776032975 @default.
- W2010600019 hasConcept C2908647359 @default.
- W2010600019 hasConcept C29331040 @default.
- W2010600019 hasConcept C37218471 @default.
- W2010600019 hasConcept C47785574 @default.
- W2010600019 hasConcept C50738837 @default.
- W2010600019 hasConcept C519063684 @default.
- W2010600019 hasConcept C71924100 @default.