Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010622172> ?p ?o ?g. }
- W2010622172 endingPage "882" @default.
- W2010622172 startingPage "861" @default.
- W2010622172 abstract "Electron crystallography has the potential to visualise the charge status of atoms. This is due to the significantly different scattering factors of neutral and ionised atoms for electrons in the low-resolution range (typically less than 5 Å). In previous work, we observed two different types of densities around acidic residues in the experimental (|Fo|) map of bacteriorhodopsin (bR), a light-driven proton pump. We suggested that these might reflect different states of the acidic residues; namely, the protonated (neutral) and the deprotonated (negatively charged) state. To evaluate the observed charge more quantitatively, we refined the atomic model for bR and eight surrounding lipids using our electron crystallographic data set between 8.0 and 3.0 Å resolution, where the charge effect is small. The refined model yielded an R-factor of 23.7 % and a free R-factor of 33.0 %. To evaluate the effect of charges on the density map, we calculated a difference (|Fo|−|Fc|) map including data of a resolution lower than 8.0 Å resolution, where the charge effect is significant. We found strong peaks in the difference map mainly in the backbone region of the transmembrane helices. We interpreted these peaks to come from the polarisation of the polar groups in the main chain of the α-helices and we examined this by assuming a partial charge of 0.5 for the peptide carbonyl groups. The resulting R and free R-factors dropped from 0.250 and 0.341 to 0.246 and 0.336, respectively. Furthermore, we also observed some strong peaks around some side-chains, which could be assigned to positively charged atoms. Thus, we could show that Asp36 and Asp102 are likely to interact with cations nearby. In addition, peaks found around the acidic residues Glu74, Glu194 and Glu212 have different features and might represent positive charges on polarised water molecules or hydroxonium ions." @default.
- W2010622172 created "2016-06-24" @default.
- W2010622172 creator A5006218247 @default.
- W2010622172 creator A5021942420 @default.
- W2010622172 creator A5022487976 @default.
- W2010622172 creator A5032548893 @default.
- W2010622172 creator A5043817262 @default.
- W2010622172 creator A5061390903 @default.
- W2010622172 creator A5000446115 @default.
- W2010622172 date "1999-02-01" @default.
- W2010622172 modified "2023-10-11" @default.
- W2010622172 title "The structure of bacteriorhodopsin at 3.0 Å resolution based on electron crystallography: implication of the charge distribution 1 1Edited by R. Huber" @default.
- W2010622172 cites W1499129928 @default.
- W2010622172 cites W1500770275 @default.
- W2010622172 cites W18007467 @default.
- W2010622172 cites W1968568101 @default.
- W2010622172 cites W1974402242 @default.
- W2010622172 cites W1975914851 @default.
- W2010622172 cites W1976422615 @default.
- W2010622172 cites W1978648048 @default.
- W2010622172 cites W1978886142 @default.
- W2010622172 cites W1986191025 @default.
- W2010622172 cites W2001641653 @default.
- W2010622172 cites W2001920088 @default.
- W2010622172 cites W2003618021 @default.
- W2010622172 cites W2008708467 @default.
- W2010622172 cites W2012760852 @default.
- W2010622172 cites W2014400817 @default.
- W2010622172 cites W2019017134 @default.
- W2010622172 cites W2028231353 @default.
- W2010622172 cites W2031593490 @default.
- W2010622172 cites W2040717718 @default.
- W2010622172 cites W2042089535 @default.
- W2010622172 cites W2044082153 @default.
- W2010622172 cites W2048695489 @default.
- W2010622172 cites W2049510427 @default.
- W2010622172 cites W2054288728 @default.
- W2010622172 cites W2057014616 @default.
- W2010622172 cites W2062021082 @default.
- W2010622172 cites W2068037979 @default.
- W2010622172 cites W2072070542 @default.
- W2010622172 cites W2072414401 @default.
- W2010622172 cites W2078248419 @default.
- W2010622172 cites W2082825017 @default.
- W2010622172 cites W2093650342 @default.
- W2010622172 cites W2110810808 @default.
- W2010622172 cites W2116275717 @default.
- W2010622172 cites W2122109248 @default.
- W2010622172 cites W2159477987 @default.
- W2010622172 cites W2160115625 @default.
- W2010622172 cites W2161743998 @default.
- W2010622172 cites W2162166182 @default.
- W2010622172 cites W959729437 @default.
- W2010622172 doi "https://doi.org/10.1006/jmbi.1998.2529" @default.
- W2010622172 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10024456" @default.
- W2010622172 hasPublicationYear "1999" @default.
- W2010622172 type Work @default.
- W2010622172 sameAs 2010622172 @default.
- W2010622172 citedByCount "253" @default.
- W2010622172 countsByYear W20106221722012 @default.
- W2010622172 countsByYear W20106221722013 @default.
- W2010622172 countsByYear W20106221722014 @default.
- W2010622172 countsByYear W20106221722015 @default.
- W2010622172 countsByYear W20106221722016 @default.
- W2010622172 countsByYear W20106221722017 @default.
- W2010622172 countsByYear W20106221722018 @default.
- W2010622172 countsByYear W20106221722019 @default.
- W2010622172 countsByYear W20106221722020 @default.
- W2010622172 countsByYear W20106221722021 @default.
- W2010622172 countsByYear W20106221722022 @default.
- W2010622172 countsByYear W20106221722023 @default.
- W2010622172 crossrefType "journal-article" @default.
- W2010622172 hasAuthorship W2010622172A5000446115 @default.
- W2010622172 hasAuthorship W2010622172A5006218247 @default.
- W2010622172 hasAuthorship W2010622172A5021942420 @default.
- W2010622172 hasAuthorship W2010622172A5022487976 @default.
- W2010622172 hasAuthorship W2010622172A5032548893 @default.
- W2010622172 hasAuthorship W2010622172A5043817262 @default.
- W2010622172 hasAuthorship W2010622172A5061390903 @default.
- W2010622172 hasConcept C118629725 @default.
- W2010622172 hasConcept C121332964 @default.
- W2010622172 hasConcept C125485243 @default.
- W2010622172 hasConcept C138268822 @default.
- W2010622172 hasConcept C145148216 @default.
- W2010622172 hasConcept C147120987 @default.
- W2010622172 hasConcept C150708132 @default.
- W2010622172 hasConcept C154945302 @default.
- W2010622172 hasConcept C178790620 @default.
- W2010622172 hasConcept C185592680 @default.
- W2010622172 hasConcept C188082385 @default.
- W2010622172 hasConcept C189542057 @default.
- W2010622172 hasConcept C30095370 @default.
- W2010622172 hasConcept C41008148 @default.
- W2010622172 hasConcept C41625074 @default.
- W2010622172 hasConcept C54516573 @default.
- W2010622172 hasConcept C55493867 @default.
- W2010622172 hasConcept C62520636 @default.
- W2010622172 hasConcept C8010536 @default.